In utero electroporation in mice

Chunlei Wang, Lin Mei

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations

Abstract

In utero electroporation has been extensively used to study a variety of developmental questions in the developing brain. This protocol aims to provide the basic knowledge for a beginner to get familiar with the technique. Basically, by electroporating a DNA construct into a subpopulation of progenitor cells in the ventricular zone of embryonic brain, the progenitor cells carrying the DNA will undergo neurogenesis, migration, and final differentiation to become mature neurons positioned in distinct cortical layers according to their birth date. In addition, by controlling the direction of electroporation, a specific cortical area can be targeted. Thus, in utero electroporation allows gene modification in a specific cortical layer in a specific cortical area.

Original languageEnglish (US)
Title of host publicationNeural Development
Subtitle of host publicationMethods and Protocols
Pages151-163
Number of pages13
DOIs
StatePublished - Sep 4 2013

Publication series

NameMethods in Molecular Biology
Volume1018
ISSN (Print)1064-3745

Keywords

  • Brain development
  • Gene modification
  • In utero electroporation
  • Mouse embryos
  • Plasmid DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Wang, C., & Mei, L. (2013). In utero electroporation in mice. In Neural Development: Methods and Protocols (pp. 151-163). (Methods in Molecular Biology; Vol. 1018). https://doi.org/10.1007/978-1-62703-444-9-15