In vitro and in vivo evidences that antioxidant action contributes to the neuroprotective effects of the neuronal nitric oxide synthase and monoamine oxidase-B inhibitor, 7-nitroindazole

Bobby Thomas, Karuppagounder S. Saravanan, Kochupurackal P. Mohanakumar

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

The neuronal nitric oxide synthase (nNOS) inhibitor, 7-nitroindazole (7-NI) is neuroprotective against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced parkinsonism. Monoamine oxidase (MAO)-B inhibitory action partially contributes to this effect. We tested the hypothesis that 7-NI could be a powerful hydroxyl radical ({radical dot}OH) scavenger, and interferes with oxidative stress caused by MPTP. We measured {radical dot}OH, reduced glutathione (GSH), as well as superoxide dismutase (SOD) and catalase activities in the nucleus caudatus putamen and substantia nigra of Balb/c mice following MPTP and/or 7-NI administration. The nNOS inhibitor caused dose-dependent inhibition in the production of {radical dot}OH in (i) Fenton-like reaction employing ferrous citrate in a cell-free system in test tubes, (ii) in isolated mitochondrial preparation in presence of MPP+, and (iii) in the striatum of mice systemically treated with MPTP. An MPTP-induced depletion of GSH in both the nuclei was blocked by 7-NI, which was dose-dependent (10-50 mg/kg), but independent of MAO-B inhibition. The nNOS-mediated recovery of GSH paralleled attenuation of MPTP-induced depletion of striatal dopamine. MPTP-induced increase in the activities of striatal or nigral SOD and catalase were significantly attenuated by 7-NI treatment. These results suggest potent antioxidant action of 7-NI in its neuroprotective effects against MPTP-induced neurotoxicity.

Original languageEnglish (US)
Pages (from-to)990-1001
Number of pages12
JournalNeurochemistry International
Volume52
Issue number6
DOIs
StatePublished - May 1 2008

Keywords

  • Antioxidant enzymes
  • Endogenous antioxidant molecules
  • Experimental Parkinson's disease
  • Ferrous citrate
  • Free radical scavenger
  • MPP-induced {radical dot}OH generation
  • Neuroprotective therapy
  • Oxidative stress
  • Striatal dopamine depletion

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

Fingerprint Dive into the research topics of 'In vitro and in vivo evidences that antioxidant action contributes to the neuroprotective effects of the neuronal nitric oxide synthase and monoamine oxidase-B inhibitor, 7-nitroindazole'. Together they form a unique fingerprint.

  • Cite this