In vitro antifungal activity of CAN-296: A naturally occurring complex carbohydrate

Avital Mazar Ben-Josef, Elias K. Manavathu, David Platt, Jack D. Sobel

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Abstract

The in vitro activity of a naturally occurring complex carbohydrate, CAN-296, was evaluated by testing 132 clinical and ATCC isolates of yeast and Aspergillus fumigatus, many of which were azole-resistant. The in vitro susceptibility tests were performed by standardized broth micro-and macrodilution methods and results were compared with those obtained for amphotericin B, fluconazole, ketoconazole, flucytosine and the pneumocandin L-733,560. All tested Candida species showed highly uniform susceptibility to CAN-296 at concentrations of 0.078 to 0.312 μg/ml; non-albicans Candida were as susceptible to CAN-296 as the Candida albicans strains. Multi-azoleresistant Candida species were highly sensitive to CAN-296. Minimum inhibitory concentration measurements did not differ from minimum lethal concentrations by more than two-fold for all tested Candida species. Aspergillus fumigatus, on the other hand, showed only moderate susceptibility to CAN-296. The kinetics of the anti-Candida activity of CAN-296 was investigated by kill-curve experiments using C. albicans and C. glabrata and the results were compared with those obtain for amphotericin B. CAN-296 was found to be rapidly fungicidal in concentrations ranging from 4~16 fold the mean MIC value. The broad spectrum of anti-Candida activity together with the rapid fungicidal effect make this complex carbohydrate a promising agent for clinical use.

Original languageEnglish (US)
Pages (from-to)937-943
Number of pages7
JournalJournal of Antibiotics
Volume50
Issue number11
DOIs
StatePublished - Nov 1997

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ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

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