In vitro biocompatibility and oxidative stress profiles of different hydraulic calcium silicate cements

Ashraf A. Eid, Johnny L. Gosier, Carolyn M. Primus, Barry Dale Hammond, Lisiane Ferreira Susin, David Henry Pashley, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Introduction MTA Plus is a new calcium silicate cement with unknown cytotoxicity characteristics. The objectives of this study were to examine the effect of MTA Plus on the viability, apoptosis/necrosis profile, and oxidative stress levels of rat odontoblast-like cells. Methods MDPC-23 cells were exposed to gray and white MTA Plus (GMTAP, WMTAP), gray and white ProRoot MTA (GMTA, WMTA) cements, or their eluents. The cells were evaluated for (1) cell viability by using XTT assay, (2) apoptosis/necrosis by using flow cytometry and confocal laser scanning microscopy, and (3) oxidative stress by measuring reactive oxygen species. Results XTT assay showed that all test cements exhibited marked initial cytotoxicity that decreased with time. By the end of the third week, GMTAP and GMTA were comparable to untreated cells (negative control) in terms of cell viability, whereas WMTAP and WMTA were significantly lower than the untreated cells. Apoptosis/necrosis profiles of cells exposed to WMTAP and GMTAP were not significantly different from untreated cells, whereas cells exposed to WMTA and GMTA showed significantly less viable cells. All experimental groups exhibited reduction of intracellular reactive oxygen species formation compared with untreated cells, although cells exposed to WMTA were not significantly different from untreated cells. Conclusions Both the gray and white versions of MTA Plus possess negligible in vitro cytotoxic risks that are time and dilution dependent. They enrich the spectrum of hydraulic calcium silicate cements currently available to clinicians for endodontic applications.

Original languageEnglish (US)
Pages (from-to)255-260
Number of pages6
JournalJournal of Endodontics
Volume40
Issue number2
DOIs
StatePublished - Feb 1 2014

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Silicate Cement
Oxidative Stress
Pemetrexed
Necrosis
Apoptosis
In Vitro Techniques
calcium silicate
Reactive Oxygen Species
Cell Survival
Odontoblasts
Endodontics

Keywords

  • Apoptosis
  • cell viability
  • hydraulic calcium silicate cements
  • necrosis
  • reactive oxygen species

ASJC Scopus subject areas

  • Dentistry(all)
  • Medicine(all)

Cite this

In vitro biocompatibility and oxidative stress profiles of different hydraulic calcium silicate cements. / Eid, Ashraf A.; Gosier, Johnny L.; Primus, Carolyn M.; Hammond, Barry Dale; Ferreira Susin, Lisiane; Pashley, David Henry; Tay, Franklin Chi Meng.

In: Journal of Endodontics, Vol. 40, No. 2, 01.02.2014, p. 255-260.

Research output: Contribution to journalArticle

Eid, Ashraf A. ; Gosier, Johnny L. ; Primus, Carolyn M. ; Hammond, Barry Dale ; Ferreira Susin, Lisiane ; Pashley, David Henry ; Tay, Franklin Chi Meng. / In vitro biocompatibility and oxidative stress profiles of different hydraulic calcium silicate cements. In: Journal of Endodontics. 2014 ; Vol. 40, No. 2. pp. 255-260.
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