Abstract
A RNA-trimming plasmid pRG523 is constructed, in which three Rz genes, GR5(5'-cis-Rz gene), HR2G(trans-Rz gene) and GR3(3'-cis-Rz gene), are arranged in the order from 5' to 3' downstream from the T7 promoter. In vitro transcription of this plasmid shows that the trans-Rz can be trimmed to definite lengths by the cis-Rz on both sides of the trans-Rz. In vitro cleavage of HPV16 E6 and E7 RNA fragments of different lengths by synthetic Rz and that of E7 RNA with a length of 171 nt by synthetic Rz and transcribed Rzs with different lengths of flanking sequences is studied. The results show that the non-base-pairing flanking sequences on both Rz and target RNA can affect the cleavage reaction.
Original language | English (US) |
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Pages (from-to) | 21-24 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 322 |
Issue number | 1 |
DOIs | |
State | Published - May 3 1993 |
Externally published | Yes |
Keywords
- Human papillomavirus type 16
- In vitro cleavage
- In vitro transcription
- RNA-trimming plasmid
- Ribozyme
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology