In vitro cleavage of HPV16 E6 and E7 RNA fragments by synthetic ribozymes and transcribed ribozymes from RNA-trimming plasmids

Yukai He, Chang De Lu, Guo Rong Qi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A RNA-trimming plasmid pRG523 is constructed, in which three Rz genes, GR5(5'-cis-Rz gene), HR2G(trans-Rz gene) and GR3(3'-cis-Rz gene), are arranged in the order from 5' to 3' downstream from the T7 promoter. In vitro transcription of this plasmid shows that the trans-Rz can be trimmed to definite lengths by the cis-Rz on both sides of the trans-Rz. In vitro cleavage of HPV16 E6 and E7 RNA fragments of different lengths by synthetic Rz and that of E7 RNA with a length of 171 nt by synthetic Rz and transcribed Rzs with different lengths of flanking sequences is studied. The results show that the non-base-pairing flanking sequences on both Rz and target RNA can affect the cleavage reaction.

Original languageEnglish (US)
Pages (from-to)21-24
Number of pages4
JournalFEBS Letters
Volume322
Issue number1
DOIs
StatePublished - May 3 1993
Externally publishedYes

Fingerprint

Catalytic RNA
Trimming
Plasmids
Genes
RNA
Transcription
In Vitro Techniques

Keywords

  • Human papillomavirus type 16
  • In vitro cleavage
  • In vitro transcription
  • RNA-trimming plasmid
  • Ribozyme

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

In vitro cleavage of HPV16 E6 and E7 RNA fragments by synthetic ribozymes and transcribed ribozymes from RNA-trimming plasmids. / He, Yukai; Lu, Chang De; Qi, Guo Rong.

In: FEBS Letters, Vol. 322, No. 1, 03.05.1993, p. 21-24.

Research output: Contribution to journalArticle

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N2 - A RNA-trimming plasmid pRG523 is constructed, in which three Rz genes, GR5(5'-cis-Rz gene), HR2G(trans-Rz gene) and GR3(3'-cis-Rz gene), are arranged in the order from 5' to 3' downstream from the T7 promoter. In vitro transcription of this plasmid shows that the trans-Rz can be trimmed to definite lengths by the cis-Rz on both sides of the trans-Rz. In vitro cleavage of HPV16 E6 and E7 RNA fragments of different lengths by synthetic Rz and that of E7 RNA with a length of 171 nt by synthetic Rz and transcribed Rzs with different lengths of flanking sequences is studied. The results show that the non-base-pairing flanking sequences on both Rz and target RNA can affect the cleavage reaction.

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