In vivo cellular imaging for translational medical research

Ali Syed Arbab, Branislava Janic, Jodi Haller, Edyta Pawelczyk, Wei Liu, Joseph A. Frank

Research output: Contribution to journalReview article

62 Citations (Scopus)

Abstract

Personalized treatment using stem, modified or genetically engineered, cells is becoming a reality in the field of medicine, in which allogenic or autologous cells can be used for treatment and possibly for early diagnosis of diseases. Hematopoietic, stromal and organ specific stem cells are under evaluation for cell-based therapies for cardiac, neurological, autoimmune and other disorders. Cytotoxic or genetically altered T-cells are under clinical trial for the treatment of hematopoietic or other malignant diseases. Before using stem cells in clinical trials, translational research in experimental animal models are essential, with a critical emphasis on developing noninvasive methods for tracking the temporal and spatial homing of these cells to target tissues. Moreover, it is necessary to determine the transplanted cells, engraftment efficiency and functional capability. Various in vivo imaging modalities are in use to track the movement and incorporation of administered cells. Tagging cells with reporter genes, fluorescent dyes or different contrast agents transforms them into cellular probes or imaging agents. Recent reports have shown that magnetically labeled cells can be used as cellular magnetic resonance imaging (MRI) probes, demonstrating the cell trafficking to target tissues. In this review, we will discuss the methods to transform cells into probes for in vivo imaging, along with their advantages and disadvantages as well as the future clinical applicability of cellular imaging method and corresponding imaging modality.

Original languageEnglish (US)
Pages (from-to)19-38
Number of pages20
JournalCurrent Medical Imaging Reviews
Volume5
Issue number1
DOIs
StatePublished - Jun 2 2009

Fingerprint

Translational Medical Research
Stem Cells
Clinical Trials
Cell- and Tissue-Based Therapy
Fluorescent Dyes
Reporter Genes
Contrast Media
Early Diagnosis
Therapeutics
Animal Models
Magnetic Resonance Imaging
Medicine
T-Lymphocytes

Keywords

  • Cell tracking
  • Cellular magnetic resonance (CMRI)
  • Magnetic cell labeling
  • SPION
  • Stem cells

ASJC Scopus subject areas

  • Internal Medicine
  • Radiology Nuclear Medicine and imaging

Cite this

In vivo cellular imaging for translational medical research. / Arbab, Ali Syed; Janic, Branislava; Haller, Jodi; Pawelczyk, Edyta; Liu, Wei; Frank, Joseph A.

In: Current Medical Imaging Reviews, Vol. 5, No. 1, 02.06.2009, p. 19-38.

Research output: Contribution to journalReview article

Arbab, AS, Janic, B, Haller, J, Pawelczyk, E, Liu, W & Frank, JA 2009, 'In vivo cellular imaging for translational medical research', Current Medical Imaging Reviews, vol. 5, no. 1, pp. 19-38. https://doi.org/10.2174/157340509787354697
Arbab, Ali Syed ; Janic, Branislava ; Haller, Jodi ; Pawelczyk, Edyta ; Liu, Wei ; Frank, Joseph A. / In vivo cellular imaging for translational medical research. In: Current Medical Imaging Reviews. 2009 ; Vol. 5, No. 1. pp. 19-38.
@article{6c84aa3647504ac8b4e7710f9f2a0f72,
title = "In vivo cellular imaging for translational medical research",
abstract = "Personalized treatment using stem, modified or genetically engineered, cells is becoming a reality in the field of medicine, in which allogenic or autologous cells can be used for treatment and possibly for early diagnosis of diseases. Hematopoietic, stromal and organ specific stem cells are under evaluation for cell-based therapies for cardiac, neurological, autoimmune and other disorders. Cytotoxic or genetically altered T-cells are under clinical trial for the treatment of hematopoietic or other malignant diseases. Before using stem cells in clinical trials, translational research in experimental animal models are essential, with a critical emphasis on developing noninvasive methods for tracking the temporal and spatial homing of these cells to target tissues. Moreover, it is necessary to determine the transplanted cells, engraftment efficiency and functional capability. Various in vivo imaging modalities are in use to track the movement and incorporation of administered cells. Tagging cells with reporter genes, fluorescent dyes or different contrast agents transforms them into cellular probes or imaging agents. Recent reports have shown that magnetically labeled cells can be used as cellular magnetic resonance imaging (MRI) probes, demonstrating the cell trafficking to target tissues. In this review, we will discuss the methods to transform cells into probes for in vivo imaging, along with their advantages and disadvantages as well as the future clinical applicability of cellular imaging method and corresponding imaging modality.",
keywords = "Cell tracking, Cellular magnetic resonance (CMRI), Magnetic cell labeling, SPION, Stem cells",
author = "Arbab, {Ali Syed} and Branislava Janic and Jodi Haller and Edyta Pawelczyk and Wei Liu and Frank, {Joseph A.}",
year = "2009",
month = "6",
day = "2",
doi = "10.2174/157340509787354697",
language = "English (US)",
volume = "5",
pages = "19--38",
journal = "Current Medical Imaging Reviews",
issn = "1573-4056",
publisher = "Bentham Science Publishers B.V.",
number = "1",

}

TY - JOUR

T1 - In vivo cellular imaging for translational medical research

AU - Arbab, Ali Syed

AU - Janic, Branislava

AU - Haller, Jodi

AU - Pawelczyk, Edyta

AU - Liu, Wei

AU - Frank, Joseph A.

PY - 2009/6/2

Y1 - 2009/6/2

N2 - Personalized treatment using stem, modified or genetically engineered, cells is becoming a reality in the field of medicine, in which allogenic or autologous cells can be used for treatment and possibly for early diagnosis of diseases. Hematopoietic, stromal and organ specific stem cells are under evaluation for cell-based therapies for cardiac, neurological, autoimmune and other disorders. Cytotoxic or genetically altered T-cells are under clinical trial for the treatment of hematopoietic or other malignant diseases. Before using stem cells in clinical trials, translational research in experimental animal models are essential, with a critical emphasis on developing noninvasive methods for tracking the temporal and spatial homing of these cells to target tissues. Moreover, it is necessary to determine the transplanted cells, engraftment efficiency and functional capability. Various in vivo imaging modalities are in use to track the movement and incorporation of administered cells. Tagging cells with reporter genes, fluorescent dyes or different contrast agents transforms them into cellular probes or imaging agents. Recent reports have shown that magnetically labeled cells can be used as cellular magnetic resonance imaging (MRI) probes, demonstrating the cell trafficking to target tissues. In this review, we will discuss the methods to transform cells into probes for in vivo imaging, along with their advantages and disadvantages as well as the future clinical applicability of cellular imaging method and corresponding imaging modality.

AB - Personalized treatment using stem, modified or genetically engineered, cells is becoming a reality in the field of medicine, in which allogenic or autologous cells can be used for treatment and possibly for early diagnosis of diseases. Hematopoietic, stromal and organ specific stem cells are under evaluation for cell-based therapies for cardiac, neurological, autoimmune and other disorders. Cytotoxic or genetically altered T-cells are under clinical trial for the treatment of hematopoietic or other malignant diseases. Before using stem cells in clinical trials, translational research in experimental animal models are essential, with a critical emphasis on developing noninvasive methods for tracking the temporal and spatial homing of these cells to target tissues. Moreover, it is necessary to determine the transplanted cells, engraftment efficiency and functional capability. Various in vivo imaging modalities are in use to track the movement and incorporation of administered cells. Tagging cells with reporter genes, fluorescent dyes or different contrast agents transforms them into cellular probes or imaging agents. Recent reports have shown that magnetically labeled cells can be used as cellular magnetic resonance imaging (MRI) probes, demonstrating the cell trafficking to target tissues. In this review, we will discuss the methods to transform cells into probes for in vivo imaging, along with their advantages and disadvantages as well as the future clinical applicability of cellular imaging method and corresponding imaging modality.

KW - Cell tracking

KW - Cellular magnetic resonance (CMRI)

KW - Magnetic cell labeling

KW - SPION

KW - Stem cells

UR - http://www.scopus.com/inward/record.url?scp=66049115520&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=66049115520&partnerID=8YFLogxK

U2 - 10.2174/157340509787354697

DO - 10.2174/157340509787354697

M3 - Review article

AN - SCOPUS:66049115520

VL - 5

SP - 19

EP - 38

JO - Current Medical Imaging Reviews

JF - Current Medical Imaging Reviews

SN - 1573-4056

IS - 1

ER -