Increased nucleic acid receptor expression in chronic periodontitis

S. Esra Sahingur, Xia Juan Xia, Stephanie C. Voth, William Andrew Yeudall, John C. Gunsolley

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: Nucleic acid sensing has emerged as one of the important components of the immune system triggering inflammation. The aim of this study is to determine the expression of bacterial DNA sensors, including Toll-like receptor 9 (TLR-9), DNA-dependent activator of interferonregulatory factors (DAI), and absent in melanoma 2 (AIM2) in chronic periodontitis (CP versus healthy) (H) tissues. Methods: Thirty-five CP and 27 H gingival biopsies were included. Real-time quantitative polymerase chain reaction was performed to determine mRNA levels of AIM2, DAI, and TLRs (TLR-1 through TLR-9). The difference in gene expression for each sensor between CP and H tissues was calculated using analysis of covariance. The Spearman test was used to determine correlations among innate receptors. The expression of TLR-9, AIM2, and DAI in gingival tissues was further confirmed using immunohistochemistry. Results: The present results reveal statistically significant upregulation of TLR-9 (P <0.006), DAI (P <0.001), and TLR-8 (P <0.01) in CP tissues compared to H sites. Although mRNA expression was not changed significantly between groups for other receptors, the present results reveal significant correlations between receptors (P <0.05), suggesting that cooperation between multiple components of the host immune system may influence the overall response. Immunohistochemistry further confirmed expression of TLR-9, AIM2, and DAI in gingival tissues. Conclusions: This study highlights a possible role for nucleic acid receptors in periodontal inflammation. Future investigations will determine whether cytoplasmic receptors and their ligands can be targeted to improve clinical outcomes in periodontitis. J Periodontol 2013;84:e48-e57.

Original languageEnglish (US)
JournalJournal of periodontology
Volume84
Issue number10
DOIs
StatePublished - Oct 1 2013
Externally publishedYes

Fingerprint

Toll-Like Receptor 9
Chronic Periodontitis
Nucleic Acids
Melanoma
Immune System
Immunohistochemistry
Inflammation
Bacterial DNA
Messenger RNA
Periodontitis
Cytoplasmic and Nuclear Receptors
Real-Time Polymerase Chain Reaction
Up-Regulation
Ligands
Biopsy
Gene Expression
DNA

Keywords

  • AIM2
  • Bacterial
  • DNA
  • Inflammation
  • Periodontal disease
  • Periodontitis
  • Toll-like receptors

ASJC Scopus subject areas

  • Periodontics

Cite this

Increased nucleic acid receptor expression in chronic periodontitis. / Sahingur, S. Esra; Xia, Xia Juan; Voth, Stephanie C.; Yeudall, William Andrew; Gunsolley, John C.

In: Journal of periodontology, Vol. 84, No. 10, 01.10.2013.

Research output: Contribution to journalArticle

Sahingur, S. Esra ; Xia, Xia Juan ; Voth, Stephanie C. ; Yeudall, William Andrew ; Gunsolley, John C. / Increased nucleic acid receptor expression in chronic periodontitis. In: Journal of periodontology. 2013 ; Vol. 84, No. 10.
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