Induction of anti-DNA antibodies in non autoimmune mice by immunization with a DNA-DNAase I complex

B. Marchini, A. Puccetti, M. P. Dolcher, M. P. Madaio, P. Migliorini

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Recent studies suggest that anti-DNA antibodies may arise from the immune response to a complex of DNA and a DNA-binding protein. One of the protein targets frequently recognized by anti-DNA antibodies is the enzyme DNAase I. To investigate the possible role of DNAase I in the induction of anti-DNA antibodies, we immunized mice with a complex of DNA and DNAase I. Mammalian double strand DNA was crosslinked with DNAase I by ultraviolet light (UV) treatment and emulsified in complete Freund's adjuvant. BALB/c mice were immunized at the base of the tail with the DNA-DNAase complex, boosted afer 2 weeks with the immunogen in incomplete adjuvant and bled one week after the boost. Control mice received UV treated DNA in adjuvant. In one-third of the mice immunized with the DNA-DNAase complex, IgG anti-DNA antibodies were detectable in serum; the antibodies reacted with single and double strand DNA. No anti-DNA response was elicited by immunization with DNA alone. These data show that immunization with a DNA-DNAase complex can induce anti-DNA antibodies in non-autoimmune mice strains and suggest that DNA-binding proteins may act as carriers in the immune response that leads to anti-DNA antibody production.

Original languageEnglish (US)
Pages (from-to)7-10
Number of pages4
JournalClinical and Experimental Rheumatology
Volume13
Issue number1
StatePublished - Jan 1 1995

Keywords

  • anti-DNA antibodies
  • autoantigens
  • systemic lupus

ASJC Scopus subject areas

  • Rheumatology
  • Immunology and Allergy
  • Immunology

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    Marchini, B., Puccetti, A., Dolcher, M. P., Madaio, M. P., & Migliorini, P. (1995). Induction of anti-DNA antibodies in non autoimmune mice by immunization with a DNA-DNAase I complex. Clinical and Experimental Rheumatology, 13(1), 7-10.