Induction of murine hepatic glutathione S-transferase by dietary dehydroepiandrosterone

Leon Milewich, Mario Marrero, Bilquis U. Tezabwala, Michael Bennett, Rene A. Frenkel, Clive A. Slaughter

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The naturally occurring steroid dehydroepiandrosterone (DHEA), when administered as a supplement to the diet of mice and rats, produces alterations in the relative concentrations of specific liver proteins; among these, a protein of Mr ∼ 28 K is markedly induced by DHEA action. In the present study we identified the murine hepatic ∼ 28 kDa protein as glutathione S-transferase subtype GT-8.7. Glutathione S-transferases belong to a gene superfamily that encode closely related proteins which are induced in liver and other tissues by various chemicals, including carcinogens and chemoprotective agents such as dietary antioxidants. Based on the above finding, we evaluated glutathione S-transferase activity in cytosols and microsomes prepared from liver tissue of mice fed either a control diet or a DHEA-containing diet (0.45%, by weight). The specific activity of hepatic cytosolic glutathione S-transferase in mice treated with DHEA up to 7 days was either unchanged or slightly decreased when compared to controls; however, treatment for 14 days or longer resulted in significant increases in activity. The specific activity of microsomal glutathione S-transferase also was increased by long-term DHEA treatment; however, its activity was approximately one-tenth of that in corresponding cytosols.

Original languageEnglish (US)
Pages (from-to)321-329
Number of pages9
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume46
Issue number3
DOIs
StatePublished - Sep 1993
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

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