Inflammatory stimuli acutely modulate peripheral taste function

Devaki Kumarhia, Lianying He, Lynnette McCluskey

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Inflammation-mediated changes in taste perception can affect health outcomes in patients, but little is known about the underlying mechanisms. In the present work, we hypothesized that proinflammatory cytokines directly modulate Na+ transport in taste buds. To test this, we measured acute changes in Na+ flux in polarized fungiform taste buds loaded with a Na+ indicator dye. IL-1β elicited an amiloridesensitive increase in Na+ transport in taste buds. In contrast, TNF-α dramatically and reversibly decreased Na+ flux in polarized taste buds via amiloride-sensitive and amiloride-insensitive Na+ transport systems. The speed and partial amiloride sensitivity of these changes in Na+ flux indicate that IL-1β and TNF-α modulate epithelial Na+ channel (ENaC) function. A portion of the TNF-mediated decrease in Na+ flux is also blocked by the TRPV1 antagonist capsazepine, although TNF-α further reduced Na+ transport independently of both amiloride and capsazepine. We also assessed taste function in vivo in a model of infection and inflammation that elevates these and additional cytokines. In rats administered systemic lipopolysaccharide (LPS), CT responses to Na+ were significantly elevated between 1 and 2 h after LPS treatment. Low, normally preferred concentrations of NaCl and sodium acetate elicited high response magnitudes. Consistent with this outcome, codelivery of IL-1β and TNF-α enhanced Na+ flux in polarized taste buds. These results demonstrate that inflammation elicits swift changes in Na+ taste function, which may limit salt consumption during illness.

Original languageEnglish (US)
Pages (from-to)2964-2975
Number of pages12
JournalJournal of Neurophysiology
Volume115
Issue number6
DOIs
StatePublished - Jun 1 2016

Fingerprint

Taste Buds
Amiloride
Interleukin-1
Inflammation
Lipopolysaccharides
Taste Perception
Cytokines
Epithelial Sodium Channels
Sodium Acetate
Coloring Agents
Salts
Health
Infection

Keywords

  • Epithelial sodium channel
  • Interleukin-1β
  • Neuroimmunology
  • Taste bud
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology

Cite this

Inflammatory stimuli acutely modulate peripheral taste function. / Kumarhia, Devaki; He, Lianying; McCluskey, Lynnette.

In: Journal of Neurophysiology, Vol. 115, No. 6, 01.06.2016, p. 2964-2975.

Research output: Contribution to journalArticle

Kumarhia, Devaki ; He, Lianying ; McCluskey, Lynnette. / Inflammatory stimuli acutely modulate peripheral taste function. In: Journal of Neurophysiology. 2016 ; Vol. 115, No. 6. pp. 2964-2975.
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