Inflammatory suppression by endodontic sealers after aging 12 weeks in vitro

Martha G Brackett, Aksana Marshall, Petra E. Lockwood, Jill B. Lewis, Regina L W Messer, Serge Bouillaguet, John C. Wataha

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Dental endodontic sealers are in intimate contact with tissues around the root apex (periapical area) for extended periods. New endodontic sealers have been developed in the past decade, but the biological responses to many new products are not well documented. In this study, we assessed in vitro monocytic cytotoxic and inflammatory responses to several contemporary endodontic sealers. AH-Plus (AH), Pulp Canal Sealer (PC), Epiphany (EPH), Endo-Rez (ER), and an experimental Endo-Rez (ERx) were initially placed in buffered-saline for 12 weeks to simulate in vivo use. After "aging," specimens were placed in direct contact with THP1 monocytes for 72 h and their cytotoxicity (mitochondrial response; MTT) or ability to trigger or suppress cytokine secretion (ELISA; TNFα, IL1β, IL=6; +/- lipopolysaccharide (LPS) exposure) were measured relative to Teflon® (Tf) negative controls. Cellular responses among conditions were compared with ANOVA and Tukey post-hoc analysis (α = 0.05). Two of the five sealers, EPH and PC, still suppressed cell mitochondrial activity by 70% or more after 12 weeks of conditioning in saline. No sealer alone activated monocytic TNFα, IL1β, or IL6 secretion (p > 0.05 vs. +LPS controls). When THP1 were activated by LPS after exposure to the sealers, differential suppression of TNFα, IL1β, and IL6 secretion was observed for two of the five sealers tested. (EPH and PC) This data suggest that common endodontic sealers do not activate monocytic TNFα, IL1β, and IL6 secretion in vitro by themselves, but degradation products of the sealers may suppress activation of monocytes.

Original languageEnglish (US)
Pages (from-to)839-844
Number of pages6
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume91
Issue number2
DOIs
StatePublished - Nov 1 2009

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Endodontics
Canals
Pulp
Dental Pulp Cavity
Interleukin-6
Aging of materials
Lipopolysaccharides
Monocytes
Cytotoxicity
Analysis of variance (ANOVA)
Polytetrafluoroethylenes
Polytetrafluoroethylene
Chemical activation
Tissue
Degradation
Analysis of Variance
Tooth
Enzyme-Linked Immunosorbent Assay
Cytokines
In Vitro Techniques

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Inflammatory suppression by endodontic sealers after aging 12 weeks in vitro. / Brackett, Martha G; Marshall, Aksana; Lockwood, Petra E.; Lewis, Jill B.; Messer, Regina L W; Bouillaguet, Serge; Wataha, John C.

In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 91, No. 2, 01.11.2009, p. 839-844.

Research output: Contribution to journalArticle

Brackett, Martha G ; Marshall, Aksana ; Lockwood, Petra E. ; Lewis, Jill B. ; Messer, Regina L W ; Bouillaguet, Serge ; Wataha, John C. / Inflammatory suppression by endodontic sealers after aging 12 weeks in vitro. In: Journal of Biomedical Materials Research - Part B Applied Biomaterials. 2009 ; Vol. 91, No. 2. pp. 839-844.
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