TY - JOUR
T1 - Inflammatory suppression by endodontic sealers after aging 12 weeks in vitro
AU - Brackett, Martha Goël
AU - Marshall, Aksana
AU - Lockwood, Petra E.
AU - Lewis, Jill B.
AU - Messer, Regina L.W.
AU - Bouillaguet, Serge
AU - Wataha, John C.
PY - 2009/11
Y1 - 2009/11
N2 - Dental endodontic sealers are in intimate contact with tissues around the root apex (periapical area) for extended periods. New endodontic sealers have been developed in the past decade, but the biological responses to many new products are not well documented. In this study, we assessed in vitro monocytic cytotoxic and inflammatory responses to several contemporary endodontic sealers. AH-Plus (AH), Pulp Canal Sealer (PC), Epiphany (EPH), Endo-Rez (ER), and an experimental Endo-Rez (ERx) were initially placed in buffered-saline for 12 weeks to simulate in vivo use. After "aging," specimens were placed in direct contact with THP1 monocytes for 72 h and their cytotoxicity (mitochondrial response; MTT) or ability to trigger or suppress cytokine secretion (ELISA; TNFα, IL1β, IL=6; +/- lipopolysaccharide (LPS) exposure) were measured relative to Teflon® (Tf) negative controls. Cellular responses among conditions were compared with ANOVA and Tukey post-hoc analysis (α = 0.05). Two of the five sealers, EPH and PC, still suppressed cell mitochondrial activity by 70% or more after 12 weeks of conditioning in saline. No sealer alone activated monocytic TNFα, IL1β, or IL6 secretion (p > 0.05 vs. +LPS controls). When THP1 were activated by LPS after exposure to the sealers, differential suppression of TNFα, IL1β, and IL6 secretion was observed for two of the five sealers tested. (EPH and PC) This data suggest that common endodontic sealers do not activate monocytic TNFα, IL1β, and IL6 secretion in vitro by themselves, but degradation products of the sealers may suppress activation of monocytes.
AB - Dental endodontic sealers are in intimate contact with tissues around the root apex (periapical area) for extended periods. New endodontic sealers have been developed in the past decade, but the biological responses to many new products are not well documented. In this study, we assessed in vitro monocytic cytotoxic and inflammatory responses to several contemporary endodontic sealers. AH-Plus (AH), Pulp Canal Sealer (PC), Epiphany (EPH), Endo-Rez (ER), and an experimental Endo-Rez (ERx) were initially placed in buffered-saline for 12 weeks to simulate in vivo use. After "aging," specimens were placed in direct contact with THP1 monocytes for 72 h and their cytotoxicity (mitochondrial response; MTT) or ability to trigger or suppress cytokine secretion (ELISA; TNFα, IL1β, IL=6; +/- lipopolysaccharide (LPS) exposure) were measured relative to Teflon® (Tf) negative controls. Cellular responses among conditions were compared with ANOVA and Tukey post-hoc analysis (α = 0.05). Two of the five sealers, EPH and PC, still suppressed cell mitochondrial activity by 70% or more after 12 weeks of conditioning in saline. No sealer alone activated monocytic TNFα, IL1β, or IL6 secretion (p > 0.05 vs. +LPS controls). When THP1 were activated by LPS after exposure to the sealers, differential suppression of TNFα, IL1β, and IL6 secretion was observed for two of the five sealers tested. (EPH and PC) This data suggest that common endodontic sealers do not activate monocytic TNFα, IL1β, and IL6 secretion in vitro by themselves, but degradation products of the sealers may suppress activation of monocytes.
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U2 - 10.1002/jbm.b.31465
DO - 10.1002/jbm.b.31465
M3 - Article
C2 - 19572299
AN - SCOPUS:71649105251
SN - 1552-4973
VL - 91
SP - 839
EP - 844
JO - Journal of Biomedical Materials Research - Part B Applied Biomaterials
JF - Journal of Biomedical Materials Research - Part B Applied Biomaterials
IS - 2
ER -