Influence of carbon and nitrogen sources on glutathione catabolic enzymes in Candida albicans during dimorphism

Suresh Gunasekaran, Munyaradzi Imbayagwo, Louise McDonald, Muthukumaran Gunasekaran, Elias Manavathu

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11 Scopus citations

Abstract

The effect of carbon sources, glucose and sucrose, and nitrogen sources such as ammonia, glutamate and l-citrulline on the activities of glutathione metabolic enzymes has been studied. Yeast and mycelial cells were used to identify changes in activity levels of glutathione reductase (GSSGR), glutathione transferase (GST), glutathione peroxidase (GPX) and γ-glutamyl transpeptidase (GGT). Enzyme activities from cells grown in sucrose media were lower than in glucose media regardless of the enzyme tested, morphological form, or the growth interval. In all enzymes except GST, activity was higher in yeast form than in mycelia, regardless of nitrogen source, with lower activity from 24 to 72 h than at 96 h. In citrulline media, yeast form showed the maximum GST, GGT, and GPX activity. In ammonia-amended media, mycelia showed maximum activity in GGT, whereas in glutamate media, mycelia showed the maximum activity in GST. Also, the type of nitrogen source had no effect on GPX activity in the mycelial form. Finally, changing the nitrogen source showed no significant effect on GSSGR activity, either in the yeast or mycelial form.

Original languageEnglish (US)
Pages (from-to)93-97
Number of pages5
JournalMycopathologia
Volume131
Issue number2
DOIs
Publication statusPublished - Aug 1 1995

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Keywords

  • Candida
  • Dimorphism
  • Glutathione
  • Glutathione S-transferase
  • Glutathione peroxidase
  • Glutathione reductase
  • γ-Glutamyl transpeptidase

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Agronomy and Crop Science
  • veterinary (miscalleneous)

Cite this

Gunasekaran, S., Imbayagwo, M., McDonald, L., Gunasekaran, M., & Manavathu, E. (1995). Influence of carbon and nitrogen sources on glutathione catabolic enzymes in Candida albicans during dimorphism. Mycopathologia, 131(2), 93-97. https://doi.org/10.1007/BF01102885