Information behavior of microsatellite loci in genome scanning

W. Yuan, Hongyan Xu, J. Zhao, W. Ding, H. Jiang, M. Gu, J. Xue, J. Chen, F. Fang, Z. Chen, L. Jin, W. Huang

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Objective: To determine the heterozygosity and polymorphism information content of 139 microsatellite loci in Han population. Methods: Multiplex approach was used to analyze the 139 loci. The amplified fragments were subjected to electrophoresis in PAGE gel and analyzed with Genescan(TM) and Genotyper(TM). Results: The heterozygosities are between 0.35 to 0.89, with the heterozygosites of 88% loci >0.60. PICs are in the range of 0.32 and 0.88, with PICs of 95% loci> 0.50. Conclusion: The polymorphism information content of microsatellite loci is very high and their distribution varies in different races and populations.

Original languageEnglish (US)
Pages (from-to)65-71
Number of pages7
JournalChinese Journal of Medical Genetics
Volume17
Issue number2
StatePublished - May 4 2000
Externally publishedYes

Keywords

  • Heterozygosity
  • Microsatellite locus
  • Polymerase chain reaction
  • Polymorphism information content

ASJC Scopus subject areas

  • Genetics(clinical)

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  • Cite this

    Yuan, W., Xu, H., Zhao, J., Ding, W., Jiang, H., Gu, M., Xue, J., Chen, J., Fang, F., Chen, Z., Jin, L., & Huang, W. (2000). Information behavior of microsatellite loci in genome scanning. Chinese Journal of Medical Genetics, 17(2), 65-71.