Interleukin-10 (-819 C/T) and tumor necrosis factor-alpha (-308 G/A) gene variants influence gastritis and lymphoid follicle development

B R Achyut, Priya Tripathi, Uday Chand Ghoshal, Nikhil Moorchung, Balraj Mittal

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Helicobacter pylori (H. pylori) causes gastritis, development of lymphoid follicles and later monoclonal mucosa-associated lymphoid tissue (MALT) lymphoma. We evaluated the association of tumor necrosis factor (TNF)-alpha (-308 G/A) and IL-10 (-819 C/T) gene polymorphisms with gastritis and lymphoid follicle formation. H. pylori infection was detected using modified Giemsa staining and IgG anti-CagA enzyme-linked immunosorbent assay (ELISA). One hundred and thirty patients with non-ulcer dyspepsia (NUD) and 200 healthy age-matched controls were genotyped for TNF-alpha and IL-10 polymorphisms using polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP). Subjects with IL-10 -819 T allele [patients (46.5%) versus controls (35.7%), p = 0.006, OR = 1.56, 95% CI = 1.14-2.15] were at risk of gastritis. Infection with H. pylori was more often associated with lymphoid follicles formation than its absence (46% versus 22%, p = 0.009). TNF-alpha polymorphism did not influence gastritis but patients with TNF-alpha -308 A allele carriers showed >2 fold risk of lymphoid follicle formation [presence (26%) versus absence (11.25%), p = 0.029, OR = 2.8; 95% CI = 1.09-7.08]. There was a trend towards association of lymphoid follicles and TNF-alpha -308 A allele carriers with H. pylori infection than without (58.5% versus 22.2%; p = 0.064). IL-10 -819 T and TNF-alpha -308 A alleles may increase risk of gastritis and lymphoid follicle formation.

Original languageEnglish (US)
Pages (from-to)622-9
Number of pages8
JournalDigestive Diseases and Sciences
Volume53
Issue number3
DOIs
StatePublished - Mar 2008
Externally publishedYes

Fingerprint

Gastritis
Interleukin-10
Tumor Necrosis Factor-alpha
Helicobacter pylori
Alleles
Genes
Helicobacter Infections
Marginal Zone B-Cell Lymphoma
Dyspepsia
Restriction Fragment Length Polymorphisms
Enzyme-Linked Immunosorbent Assay
Staining and Labeling
Polymerase Chain Reaction
Infection

Keywords

  • Adult
  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Proteins
  • Case-Control Studies
  • Female
  • Gastric Mucosa
  • Gastritis
  • Helicobacter Infections
  • Helicobacter pylori
  • Humans
  • Interleukin-10
  • Lymphocytes
  • Lymphoma, B-Cell, Marginal Zone
  • Male
  • Metaplasia
  • Middle Aged
  • Neutrophils
  • Polymorphism, Single Nucleotide
  • Stomach Neoplasms
  • Tumor Necrosis Factor-alpha
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Interleukin-10 (-819 C/T) and tumor necrosis factor-alpha (-308 G/A) gene variants influence gastritis and lymphoid follicle development. / Achyut, B R; Tripathi, Priya; Ghoshal, Uday Chand; Moorchung, Nikhil; Mittal, Balraj.

In: Digestive Diseases and Sciences, Vol. 53, No. 3, 03.2008, p. 622-9.

Research output: Contribution to journalArticle

Achyut, B R ; Tripathi, Priya ; Ghoshal, Uday Chand ; Moorchung, Nikhil ; Mittal, Balraj. / Interleukin-10 (-819 C/T) and tumor necrosis factor-alpha (-308 G/A) gene variants influence gastritis and lymphoid follicle development. In: Digestive Diseases and Sciences. 2008 ; Vol. 53, No. 3. pp. 622-9.
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abstract = "Helicobacter pylori (H. pylori) causes gastritis, development of lymphoid follicles and later monoclonal mucosa-associated lymphoid tissue (MALT) lymphoma. We evaluated the association of tumor necrosis factor (TNF)-alpha (-308 G/A) and IL-10 (-819 C/T) gene polymorphisms with gastritis and lymphoid follicle formation. H. pylori infection was detected using modified Giemsa staining and IgG anti-CagA enzyme-linked immunosorbent assay (ELISA). One hundred and thirty patients with non-ulcer dyspepsia (NUD) and 200 healthy age-matched controls were genotyped for TNF-alpha and IL-10 polymorphisms using polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP). Subjects with IL-10 -819 T allele [patients (46.5{\%}) versus controls (35.7{\%}), p = 0.006, OR = 1.56, 95{\%} CI = 1.14-2.15] were at risk of gastritis. Infection with H. pylori was more often associated with lymphoid follicles formation than its absence (46{\%} versus 22{\%}, p = 0.009). TNF-alpha polymorphism did not influence gastritis but patients with TNF-alpha -308 A allele carriers showed >2 fold risk of lymphoid follicle formation [presence (26{\%}) versus absence (11.25{\%}), p = 0.029, OR = 2.8; 95{\%} CI = 1.09-7.08]. There was a trend towards association of lymphoid follicles and TNF-alpha -308 A allele carriers with H. pylori infection than without (58.5{\%} versus 22.2{\%}; p = 0.064). IL-10 -819 T and TNF-alpha -308 A alleles may increase risk of gastritis and lymphoid follicle formation.",
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AB - Helicobacter pylori (H. pylori) causes gastritis, development of lymphoid follicles and later monoclonal mucosa-associated lymphoid tissue (MALT) lymphoma. We evaluated the association of tumor necrosis factor (TNF)-alpha (-308 G/A) and IL-10 (-819 C/T) gene polymorphisms with gastritis and lymphoid follicle formation. H. pylori infection was detected using modified Giemsa staining and IgG anti-CagA enzyme-linked immunosorbent assay (ELISA). One hundred and thirty patients with non-ulcer dyspepsia (NUD) and 200 healthy age-matched controls were genotyped for TNF-alpha and IL-10 polymorphisms using polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP). Subjects with IL-10 -819 T allele [patients (46.5%) versus controls (35.7%), p = 0.006, OR = 1.56, 95% CI = 1.14-2.15] were at risk of gastritis. Infection with H. pylori was more often associated with lymphoid follicles formation than its absence (46% versus 22%, p = 0.009). TNF-alpha polymorphism did not influence gastritis but patients with TNF-alpha -308 A allele carriers showed >2 fold risk of lymphoid follicle formation [presence (26%) versus absence (11.25%), p = 0.029, OR = 2.8; 95% CI = 1.09-7.08]. There was a trend towards association of lymphoid follicles and TNF-alpha -308 A allele carriers with H. pylori infection than without (58.5% versus 22.2%; p = 0.064). IL-10 -819 T and TNF-alpha -308 A alleles may increase risk of gastritis and lymphoid follicle formation.

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KW - Polymorphism, Single Nucleotide

KW - Stomach Neoplasms

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