Abstract
Helicobacter pylori (H. pylori) causes gastritis, development of lymphoid follicles and later monoclonal mucosa-associated lymphoid tissue (MALT) lymphoma. We evaluated the association of tumor necrosis factor (TNF)-alpha (-308 G/A) and IL-10 (-819 C/T) gene polymorphisms with gastritis and lymphoid follicle formation. H. pylori infection was detected using modified Giemsa staining and IgG anti-CagA enzyme-linked immunosorbent assay (ELISA). One hundred and thirty patients with non-ulcer dyspepsia (NUD) and 200 healthy age-matched controls were genotyped for TNF-alpha and IL-10 polymorphisms using polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP). Subjects with IL-10 -819 T allele [patients (46.5%) versus controls (35.7%), p = 0.006, OR = 1.56, 95% CI = 1.14-2.15] were at risk of gastritis. Infection with H. pylori was more often associated with lymphoid follicles formation than its absence (46% versus 22%, p = 0.009). TNF-alpha polymorphism did not influence gastritis but patients with TNF-alpha -308 A allele carriers showed >2 fold risk of lymphoid follicle formation [presence (26%) versus absence (11.25%), p = 0.029, OR = 2.8; 95% CI = 1.09-7.08]. There was a trend towards association of lymphoid follicles and TNF-alpha -308 A allele carriers with H. pylori infection than without (58.5% versus 22.2%; p = 0.064). IL-10 -819 T and TNF-alpha -308 A alleles may increase risk of gastritis and lymphoid follicle formation.
Original language | English (US) |
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Pages (from-to) | 622-9 |
Number of pages | 8 |
Journal | Digestive Diseases and Sciences |
Volume | 53 |
Issue number | 3 |
DOIs | |
State | Published - Mar 2008 |
Externally published | Yes |
Keywords
- Adult
- Antibodies, Bacterial
- Antigens, Bacterial
- Bacterial Proteins
- Case-Control Studies
- Female
- Gastric Mucosa
- Gastritis
- Helicobacter Infections
- Helicobacter pylori
- Humans
- Interleukin-10
- Lymphocytes
- Lymphoma, B-Cell, Marginal Zone
- Male
- Metaplasia
- Middle Aged
- Neutrophils
- Polymorphism, Single Nucleotide
- Stomach Neoplasms
- Tumor Necrosis Factor-alpha
- Journal Article
- Research Support, Non-U.S. Gov't