Interleukin-10 (-819 C/T) and tumor necrosis factor-alpha (-308 G/A) gene variants influence gastritis and lymphoid follicle development

B R Achyut, Priya Tripathi, Uday Chand Ghoshal, Nikhil Moorchung, Balraj Mittal

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Helicobacter pylori (H. pylori) causes gastritis, development of lymphoid follicles and later monoclonal mucosa-associated lymphoid tissue (MALT) lymphoma. We evaluated the association of tumor necrosis factor (TNF)-alpha (-308 G/A) and IL-10 (-819 C/T) gene polymorphisms with gastritis and lymphoid follicle formation. H. pylori infection was detected using modified Giemsa staining and IgG anti-CagA enzyme-linked immunosorbent assay (ELISA). One hundred and thirty patients with non-ulcer dyspepsia (NUD) and 200 healthy age-matched controls were genotyped for TNF-alpha and IL-10 polymorphisms using polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP). Subjects with IL-10 -819 T allele [patients (46.5%) versus controls (35.7%), p = 0.006, OR = 1.56, 95% CI = 1.14-2.15] were at risk of gastritis. Infection with H. pylori was more often associated with lymphoid follicles formation than its absence (46% versus 22%, p = 0.009). TNF-alpha polymorphism did not influence gastritis but patients with TNF-alpha -308 A allele carriers showed >2 fold risk of lymphoid follicle formation [presence (26%) versus absence (11.25%), p = 0.029, OR = 2.8; 95% CI = 1.09-7.08]. There was a trend towards association of lymphoid follicles and TNF-alpha -308 A allele carriers with H. pylori infection than without (58.5% versus 22.2%; p = 0.064). IL-10 -819 T and TNF-alpha -308 A alleles may increase risk of gastritis and lymphoid follicle formation.

Original languageEnglish (US)
Pages (from-to)622-9
Number of pages8
JournalDigestive Diseases and Sciences
Volume53
Issue number3
DOIs
StatePublished - Mar 2008
Externally publishedYes

Keywords

  • Adult
  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Proteins
  • Case-Control Studies
  • Female
  • Gastric Mucosa
  • Gastritis
  • Helicobacter Infections
  • Helicobacter pylori
  • Humans
  • Interleukin-10
  • Lymphocytes
  • Lymphoma, B-Cell, Marginal Zone
  • Male
  • Metaplasia
  • Middle Aged
  • Neutrophils
  • Polymorphism, Single Nucleotide
  • Stomach Neoplasms
  • Tumor Necrosis Factor-alpha
  • Journal Article
  • Research Support, Non-U.S. Gov't

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