Internalization dissociates β2-adrenergic receptors

Tien Hung Lan, Sudhakiranmayi Kuravi, Nevin A. Lambert

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

G protein-coupled receptors (GPCRs) self-associate as dimers or higher-order oligomers in living cells. The stability of associated GPCRs has not been extensively studied, but it is generally thought that these receptors move between the plasma membrane and intracellular compartments as intact dimers or oligomers. Here we show that β2-adrenergic receptors (β2ARs) that self-associate at the plasma membrane can dissociate during agonist-induced internalization. We use bioluminescence-resonance energy transfer (BRET) to monitor movement of β2ARs between subcellular compartments. BRET between β2ARs and plasma membrane markers decreases in response to agonist activation, while at the same time BRET between β2ARs and endosome markers increases. Energy transfer between β2ARs is decreased in a similar manner if either the donor- or acceptor-labeled receptor is mutated to impair agonist binding and internalization. These changes take place over the course of 30 minutes, persist after agonist is removed, and are sensitive to several inhibitors of arrestin- and clathrin-mediated endocytosis. The magnitude of the decrease in BRET between donor- and acceptor-labeled β2ARs suggests that at least half of the receptors that contribute to the BRET signal are physically segregated by internalization. These results are consistent with the possibility that β2ARs associate transiently with each other in the plasma membrane, or that β2AR dimers or oligomers are actively disrupted during internalization.

Original languageEnglish (US)
Article numbere17361
JournalPloS one
Volume6
Issue number2
DOIs
StatePublished - Mar 3 2011

Fingerprint

adrenergic receptors
Bioluminescence
Energy Transfer
Adrenergic Receptors
energy transfer
bioluminescence
Energy transfer
Cell membranes
agonists
plasma membrane
Oligomers
Cell Membrane
Dimers
G-Protein-Coupled Receptors
receptors
arrestins
Arrestin
clathrin
Clathrin
endosomes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Internalization dissociates β2-adrenergic receptors. / Lan, Tien Hung; Kuravi, Sudhakiranmayi; Lambert, Nevin A.

In: PloS one, Vol. 6, No. 2, e17361, 03.03.2011.

Research output: Contribution to journalArticle

Lan, Tien Hung ; Kuravi, Sudhakiranmayi ; Lambert, Nevin A. / Internalization dissociates β2-adrenergic receptors. In: PloS one. 2011 ; Vol. 6, No. 2.
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