Ion chromatographic method for the analysis of sulfate in complex carbohydrates

H. Edward Grotjan, Paula A. Padrnos-Hicks, Brooks Allen Keel

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A procedure is described for the quantitation of sulfate in complex carbohydrates. Samples are taken to dryness in the presence of a small amount of sodium hydroxide and the sulfate is liberated by pyrolysis. Sulfate is then quantitated by single-column ion chromatography eliminating the interference by ions such as phosphate, chloride, sodium and calcium which frequently occurs with colorimetric methods. The reproducibility of the method is approximately 10%. This approach cannot be directly applied to glycoproteins because a portion of the sulfur in sulfur-containing amino acids appears to be converted to sulfate. However, this method can be used to measure sulfate in oligosaccharides of glycoproteins if the protein components are removed prior to analysis.

Original languageEnglish (US)
Pages (from-to)367-375
Number of pages9
JournalJournal of Chromatography A
Volume367
Issue numberC
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

Fingerprint

Sulfates
Chromatography
Carbohydrates
Ions
Glycoproteins
Sulfur
Sulfur Amino Acids
Sodium Hydroxide
Calcium Chloride
Ion chromatography
Oligosaccharides
Column chromatography
Sodium Chloride
Pyrolysis
Phosphates
Amino Acids
Proteins

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

Ion chromatographic method for the analysis of sulfate in complex carbohydrates. / Grotjan, H. Edward; Padrnos-Hicks, Paula A.; Keel, Brooks Allen.

In: Journal of Chromatography A, Vol. 367, No. C, 01.01.1986, p. 367-375.

Research output: Contribution to journalArticle

Grotjan, H. Edward ; Padrnos-Hicks, Paula A. ; Keel, Brooks Allen. / Ion chromatographic method for the analysis of sulfate in complex carbohydrates. In: Journal of Chromatography A. 1986 ; Vol. 367, No. C. pp. 367-375.
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