Ischemic preconditioning potentiates the protective effect of stem cells through secretion of exosomes by targeting Mecp2 via miR-22

Yuliang Feng, Wei Huang, Mashhood Wani, Xiyong Yu, Muhammad Ashraf

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSCs) have potential application for the treatment of ischemic heart diseases. Besides differentiation properties, MSCs protect ischemic cardiomyocytes by secretion of paracrine factors. In this study, we found exosomes enriched with miR-22 were secreted by MSCs following ischemic preconditioning (ExoIPC) and mobilized to cardiomyocytes where they reduced their apoptosis due to ischemia. Interestingly, by time-lapse imaging, we for the first time captured the dynamic shedding of miR-22 loaded exosomes from cytosol to extracellular space. Furthermore, the antiapoptotic effect of miR-22 was mediated by direct targeting of methyl CpG binding protein 2 (Mecp2). In vivo data showed that delivery of ExoIPC significantly reduced cardiac fibrosis. Our data identified a significant benefit of ExoIPC for the treatment of cardiac diseases by targeting Mecp2 via miR-22.

Original languageEnglish (US)
Article numbere88685
JournalPloS one
Volume9
Issue number2
DOIs
StatePublished - Feb 18 2014
Externally publishedYes

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Methyl-CpG-Binding Protein 2
exosomes
Exosomes
Ischemic Preconditioning
Stem cells
Mesenchymal Stromal Cells
protective effect
stem cells
binding proteins
Stem Cells
secretion
Cardiac Myocytes
Time-Lapse Imaging
extracellular space
Extracellular Space
heart diseases
myocardial ischemia
ischemia
fibrosis
cytosol

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Ischemic preconditioning potentiates the protective effect of stem cells through secretion of exosomes by targeting Mecp2 via miR-22. / Feng, Yuliang; Huang, Wei; Wani, Mashhood; Yu, Xiyong; Ashraf, Muhammad.

In: PloS one, Vol. 9, No. 2, e88685, 18.02.2014.

Research output: Contribution to journalArticle

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