Abstract
A novel monosialoganglioside was isolated from Tay-Sachs brains. It represented about 0.1% of the total ganglioside mixture. Compositional analysis by gas-liquid chromatography indicated that it contained glucose, galactose, N-acetylgalactosamine, N-acetylneuraminic acid, and long chain base in the molar ratio of 1:2:2:1:1. The ganglioside was found to be resistant to neuraminidase (Clostridium perfringens), β-hexosaminidase (jack bean), and β-galactosidase. However, it could be degraded by a human liver β-hexosaminidase preparation in the presence of an activator to produce a glycolipid chromatographically identical with authentic IV3NeuAc-GgOse4-ceramide. This glycolipid product was resistant to β-galactosidase (jack bean), but could be readily degraded to GgOse4-ceramide by neuraminidase. Mild formic acid hydrolysis degraded the intact ganglioside to an asialo derivative chromatographically identical with the pentahexosyl ceramide (GalNAc-Gal-GalNAc-Gal-Glc-ceramide) derived from G(D1a-GalNAc). The asialo derivative could then be degraded to GgOse4-ceramide and GgOse3-ceramide by sequential treatment with jack bean β-hexosaminidase and β-galactosidase. These data suggest that the novel ganglioside is a monosialosylpentahexosyl ceramide with the sialosyl group attached to the penultimate r224w1 =GalNAc(β1-4)Gal(β1-3)GalNAc(β1-4)Gal(β1-4)Glc-ceramide galactose moiety of the pentahexosyl ceramide backbone, and it has the following structure: (Ga1NAc(β1-4)Ga1(β1-3)Ga1NAc(β1-4)Ga1(β1-4)Glc-ceramide (3/2/α) NeuAc
Original language | English (US) |
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Pages (from-to) | 165-169 |
Number of pages | 5 |
Journal | Journal of Biological Chemistry |
Volume | 256 |
Issue number | 1 |
State | Published - 1981 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology