Isolation and characterization of a novel monosialosylpentahexosyl ceramide from Tay-Sachs brain

T. Itoh, Y. T. Li, S. C. Li, Robert K Yu

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

A novel monosialoganglioside was isolated from Tay-Sachs brains. It represented about 0.1% of the total ganglioside mixture. Compositional analysis by gas-liquid chromatography indicated that it contained glucose, galactose, N-acetylgalactosamine, N-acetylneuraminic acid, and long chain base in the molar ratio of 1:2:2:1:1. The ganglioside was found to be resistant to neuraminidase (Clostridium perfringens), β-hexosaminidase (jack bean), and β-galactosidase. However, it could be degraded by a human liver β-hexosaminidase preparation in the presence of an activator to produce a glycolipid chromatographically identical with authentic IV3NeuAc-GgOse4-ceramide. This glycolipid product was resistant to β-galactosidase (jack bean), but could be readily degraded to GgOse4-ceramide by neuraminidase. Mild formic acid hydrolysis degraded the intact ganglioside to an asialo derivative chromatographically identical with the pentahexosyl ceramide (GalNAc-Gal-GalNAc-Gal-Glc-ceramide) derived from G(D1a-GalNAc). The asialo derivative could then be degraded to GgOse4-ceramide and GgOse3-ceramide by sequential treatment with jack bean β-hexosaminidase and β-galactosidase. These data suggest that the novel ganglioside is a monosialosylpentahexosyl ceramide with the sialosyl group attached to the penultimate r224w1 =GalNAc(β1-4)Gal(β1-3)GalNAc(β1-4)Gal(β1-4)Glc-ceramide galactose moiety of the pentahexosyl ceramide backbone, and it has the following structure: (Ga1NAc(β1-4)Ga1(β1-3)Ga1NAc(β1-4)Ga1(β1-4)Glc-ceramide (3/2/α) NeuAc

Original languageEnglish (US)
Pages (from-to)165-169
Number of pages5
JournalJournal of Biological Chemistry
Volume256
Issue number1
StatePublished - Dec 1 1981
Externally publishedYes

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Ceramides
Brain
Gangliosides
Galactosidases
Hexosaminidases
Jacks
formic acid
Glycolipids
Neuraminidase
Galactose
Derivatives
Acetylgalactosamine
Clostridium
Clostridium perfringens
monosialosylpentahexosyl ceramide
Liquid chromatography
N-Acetylneuraminic Acid
Gas chromatography
Gas Chromatography
Liver

ASJC Scopus subject areas

  • Biochemistry

Cite this

Isolation and characterization of a novel monosialosylpentahexosyl ceramide from Tay-Sachs brain. / Itoh, T.; Li, Y. T.; Li, S. C.; Yu, Robert K.

In: Journal of Biological Chemistry, Vol. 256, No. 1, 01.12.1981, p. 165-169.

Research output: Contribution to journalArticle

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abstract = "A novel monosialoganglioside was isolated from Tay-Sachs brains. It represented about 0.1{\%} of the total ganglioside mixture. Compositional analysis by gas-liquid chromatography indicated that it contained glucose, galactose, N-acetylgalactosamine, N-acetylneuraminic acid, and long chain base in the molar ratio of 1:2:2:1:1. The ganglioside was found to be resistant to neuraminidase (Clostridium perfringens), β-hexosaminidase (jack bean), and β-galactosidase. However, it could be degraded by a human liver β-hexosaminidase preparation in the presence of an activator to produce a glycolipid chromatographically identical with authentic IV3NeuAc-GgOse4-ceramide. This glycolipid product was resistant to β-galactosidase (jack bean), but could be readily degraded to GgOse4-ceramide by neuraminidase. Mild formic acid hydrolysis degraded the intact ganglioside to an asialo derivative chromatographically identical with the pentahexosyl ceramide (GalNAc-Gal-GalNAc-Gal-Glc-ceramide) derived from G(D1a-GalNAc). The asialo derivative could then be degraded to GgOse4-ceramide and GgOse3-ceramide by sequential treatment with jack bean β-hexosaminidase and β-galactosidase. These data suggest that the novel ganglioside is a monosialosylpentahexosyl ceramide with the sialosyl group attached to the penultimate r224w1 =GalNAc(β1-4)Gal(β1-3)GalNAc(β1-4)Gal(β1-4)Glc-ceramide galactose moiety of the pentahexosyl ceramide backbone, and it has the following structure: (Ga1NAc(β1-4)Ga1(β1-3)Ga1NAc(β1-4)Ga1(β1-4)Glc-ceramide (3/2/α) NeuAc",
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N2 - A novel monosialoganglioside was isolated from Tay-Sachs brains. It represented about 0.1% of the total ganglioside mixture. Compositional analysis by gas-liquid chromatography indicated that it contained glucose, galactose, N-acetylgalactosamine, N-acetylneuraminic acid, and long chain base in the molar ratio of 1:2:2:1:1. The ganglioside was found to be resistant to neuraminidase (Clostridium perfringens), β-hexosaminidase (jack bean), and β-galactosidase. However, it could be degraded by a human liver β-hexosaminidase preparation in the presence of an activator to produce a glycolipid chromatographically identical with authentic IV3NeuAc-GgOse4-ceramide. This glycolipid product was resistant to β-galactosidase (jack bean), but could be readily degraded to GgOse4-ceramide by neuraminidase. Mild formic acid hydrolysis degraded the intact ganglioside to an asialo derivative chromatographically identical with the pentahexosyl ceramide (GalNAc-Gal-GalNAc-Gal-Glc-ceramide) derived from G(D1a-GalNAc). The asialo derivative could then be degraded to GgOse4-ceramide and GgOse3-ceramide by sequential treatment with jack bean β-hexosaminidase and β-galactosidase. These data suggest that the novel ganglioside is a monosialosylpentahexosyl ceramide with the sialosyl group attached to the penultimate r224w1 =GalNAc(β1-4)Gal(β1-3)GalNAc(β1-4)Gal(β1-4)Glc-ceramide galactose moiety of the pentahexosyl ceramide backbone, and it has the following structure: (Ga1NAc(β1-4)Ga1(β1-3)Ga1NAc(β1-4)Ga1(β1-4)Glc-ceramide (3/2/α) NeuAc

AB - A novel monosialoganglioside was isolated from Tay-Sachs brains. It represented about 0.1% of the total ganglioside mixture. Compositional analysis by gas-liquid chromatography indicated that it contained glucose, galactose, N-acetylgalactosamine, N-acetylneuraminic acid, and long chain base in the molar ratio of 1:2:2:1:1. The ganglioside was found to be resistant to neuraminidase (Clostridium perfringens), β-hexosaminidase (jack bean), and β-galactosidase. However, it could be degraded by a human liver β-hexosaminidase preparation in the presence of an activator to produce a glycolipid chromatographically identical with authentic IV3NeuAc-GgOse4-ceramide. This glycolipid product was resistant to β-galactosidase (jack bean), but could be readily degraded to GgOse4-ceramide by neuraminidase. Mild formic acid hydrolysis degraded the intact ganglioside to an asialo derivative chromatographically identical with the pentahexosyl ceramide (GalNAc-Gal-GalNAc-Gal-Glc-ceramide) derived from G(D1a-GalNAc). The asialo derivative could then be degraded to GgOse4-ceramide and GgOse3-ceramide by sequential treatment with jack bean β-hexosaminidase and β-galactosidase. These data suggest that the novel ganglioside is a monosialosylpentahexosyl ceramide with the sialosyl group attached to the penultimate r224w1 =GalNAc(β1-4)Gal(β1-3)GalNAc(β1-4)Gal(β1-4)Glc-ceramide galactose moiety of the pentahexosyl ceramide backbone, and it has the following structure: (Ga1NAc(β1-4)Ga1(β1-3)Ga1NAc(β1-4)Ga1(β1-4)Glc-ceramide (3/2/α) NeuAc

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