Isolation and characterization of ganglioside 9-O-acetyl-GD3 from bovine buttermilk

Daniela M. Bonafede, Lawrence J. Macala, Martha Costantine-Paton, Robert K Yu

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Bovine buttermilk contains a unique ganglioside, 9-O-acetyl-GD3. In order to isolate large quantities of this ganglioside, a simplified isolation scheme which consists of several ion-exchange and silica gel column chromatographic procedures was devised. The isolated 9-O-acetyl-GD3 was characterized on the basis of its thin-layer chromatographic behavior, its immunoreactivity with a specific monoclonal antibody, JONES, and by conversion to authentic GD3 by mild base treatment.

Original languageEnglish (US)
Pages (from-to)680-684
Number of pages5
JournalLipids
Volume24
Issue number8
DOIs
StatePublished - Aug 1 1989
Externally publishedYes

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Gangliosides
Ion Exchange
Silica Gel
Ion exchange
Monoclonal Antibodies
9-O-acetyl-GD3 ganglioside
Buttermilk

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

Cite this

Isolation and characterization of ganglioside 9-O-acetyl-GD3 from bovine buttermilk. / Bonafede, Daniela M.; Macala, Lawrence J.; Costantine-Paton, Martha; Yu, Robert K.

In: Lipids, Vol. 24, No. 8, 01.08.1989, p. 680-684.

Research output: Contribution to journalArticle

Bonafede, Daniela M. ; Macala, Lawrence J. ; Costantine-Paton, Martha ; Yu, Robert K. / Isolation and characterization of ganglioside 9-O-acetyl-GD3 from bovine buttermilk. In: Lipids. 1989 ; Vol. 24, No. 8. pp. 680-684.
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