Isolation and characterization of ganglioside 9-O-acetyl-GD3 from bovine buttermilk

Daniela M. Bonafede, Lawrence J. Macala, Martha Costantine-Paton, Robert K. Yu

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Bovine buttermilk contains a unique ganglioside, 9-O-acetyl-GD3. In order to isolate large quantities of this ganglioside, a simplified isolation scheme which consists of several ion-exchange and silica gel column chromatographic procedures was devised. The isolated 9-O-acetyl-GD3 was characterized on the basis of its thin-layer chromatographic behavior, its immunoreactivity with a specific monoclonal antibody, JONES, and by conversion to authentic GD3 by mild base treatment.

Original languageEnglish (US)
Pages (from-to)680-684
Number of pages5
JournalLipids
Volume24
Issue number8
DOIs
StatePublished - Aug 1 1989
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Isolation and characterization of ganglioside 9-O-acetyl-GD<sub>3</sub> from bovine buttermilk'. Together they form a unique fingerprint.

  • Cite this