Isolation of rat fibrillin-1 cDNA and its relevance in metanephric development

Yashpal S. Kanwar, Kosuke Ota, Qiwei Yang, Anil Kumar, Jun Wada, Naoki Kashihara, Darryl R. Peterson

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The role of fibrillin-1 in metanephrogenesis was investigated. Fibrillin-1 cDNA was isolated from the rat kidney cDNA library and sequenced, and its spatiotemporal expression was studied. It had ~88% homology with human fibrillin-1 and had Ca2+ binding epidermal growth factor-like domains, transforming growth factor-β binding protein motifs, and an RGD binding site. Northern blot analysis revealed an ~10-kb transcript, and fibrillin-1 expression was developmentally regulated. In situ hybridization and immunofluorescence studies indicated that at day 15 of gestation, fibrillin-1 is expressed in the metanephric mesenchyme. At day 18, its expression was confined to nascent blood vessels and glomeruli, and it increased in the newborn and neonatal kidneys. Immunoprecipitation revealed an ~300-kDa band by SDS-PAGE. Treatment with fibrillin-1 antisense oligodeoxynucleotide induced marked dysmorphogenesis of the embryonic metanephroi. Concomitantly, the fibrillin-1 mRNA, antibody reactivity in the metanephroi, and fibrillin-1-specific radioincorporation were reduced. These data indicate that, like αvβ3 integrin, a known morphogen and a putative receptor of fibrillin-1, the fibrillin-1 modulates events related to early organogenesis and possibly also the vascularization of the rat kidney.

Original languageEnglish (US)
Pages (from-to)F710-F723
JournalAmerican Journal of Physiology - Renal Physiology
Volume275
Issue number5 44-5
StatePublished - Nov 1 1998

Fingerprint

Complementary DNA
Kidney
Fibrillin-1
Amino Acid Motifs
Organogenesis
Oligodeoxyribonucleotides
Transforming Growth Factors
Mesoderm
Gene Library
Immunoprecipitation
Epidermal Growth Factor
Integrins
Northern Blotting
In Situ Hybridization
Fluorescent Antibody Technique
Blood Vessels
Polyacrylamide Gel Electrophoresis
Carrier Proteins
Binding Sites
Pregnancy

Keywords

  • Complementary deoxyribonucleic acid cloning
  • Extracellular matrix
  • Fibrillin

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

Kanwar, Y. S., Ota, K., Yang, Q., Kumar, A., Wada, J., Kashihara, N., & Peterson, D. R. (1998). Isolation of rat fibrillin-1 cDNA and its relevance in metanephric development. American Journal of Physiology - Renal Physiology, 275(5 44-5), F710-F723.

Isolation of rat fibrillin-1 cDNA and its relevance in metanephric development. / Kanwar, Yashpal S.; Ota, Kosuke; Yang, Qiwei; Kumar, Anil; Wada, Jun; Kashihara, Naoki; Peterson, Darryl R.

In: American Journal of Physiology - Renal Physiology, Vol. 275, No. 5 44-5, 01.11.1998, p. F710-F723.

Research output: Contribution to journalArticle

Kanwar, YS, Ota, K, Yang, Q, Kumar, A, Wada, J, Kashihara, N & Peterson, DR 1998, 'Isolation of rat fibrillin-1 cDNA and its relevance in metanephric development', American Journal of Physiology - Renal Physiology, vol. 275, no. 5 44-5, pp. F710-F723.
Kanwar YS, Ota K, Yang Q, Kumar A, Wada J, Kashihara N et al. Isolation of rat fibrillin-1 cDNA and its relevance in metanephric development. American Journal of Physiology - Renal Physiology. 1998 Nov 1;275(5 44-5):F710-F723.
Kanwar, Yashpal S. ; Ota, Kosuke ; Yang, Qiwei ; Kumar, Anil ; Wada, Jun ; Kashihara, Naoki ; Peterson, Darryl R. / Isolation of rat fibrillin-1 cDNA and its relevance in metanephric development. In: American Journal of Physiology - Renal Physiology. 1998 ; Vol. 275, No. 5 44-5. pp. F710-F723.
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