Japanese encephalitis virus nonstructural protein NS3 has RNA binding and ATPase activities

Tsutomu Takegami, Daitoku Sakamuro, Toru Furukawa

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Sequence data suggest that Japanese encephalitis virus (JEV) protein NS3 is a multifunctional protein with sequence motifs characteristic of a protease and a helicase. To examine the functions of JEV-NS3, a fusion protein of NS3 in Escherichia coli was generated. Analysis by Western blot using monospecific rabbit antisera generated against the fusion protein (anti-MBJEN3) showed that NS3 was localized in the membrane fraction of JEV-infected cells and the particulate fraction of bacteria extracts. The addition of anti-MBJEN3 sera reduced JEV-specific RNA synthesis activity in a in vitro system. In addition, NS3 was shown to exhibit RNA binding and ATPase activities, suggesting this protein has an important role in viral RNA replication in virus-infected cells.

Original languageEnglish (US)
Pages (from-to)105-112
Number of pages8
JournalVirus Genes
Volume9
Issue number2
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

Japanese Encephalitis Virus
Adenosine Triphosphatases
RNA
Proteins
Viral RNA
Virus Replication
Immune Sera
Peptide Hydrolases
Western Blotting
Escherichia coli
Rabbits
Bacteria
Membranes
flavivirus NS3 protein
Serum

Keywords

  • ATPase
  • Japanese encephalitis virus
  • RNA binding
  • expressed protein
  • nonstructural protein NS3

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

Cite this

Japanese encephalitis virus nonstructural protein NS3 has RNA binding and ATPase activities. / Takegami, Tsutomu; Sakamuro, Daitoku; Furukawa, Toru.

In: Virus Genes, Vol. 9, No. 2, 01.01.1995, p. 105-112.

Research output: Contribution to journalArticle

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