Japanese encephalitis virus nonstructural protein NS3 has RNA binding and ATPase activities

Tsutomu Takegami, Daitoku Sakamuro, Toru Furukawa

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

Sequence data suggest that Japanese encephalitis virus (JEV) protein NS3 is a multifunctional protein with sequence motifs characteristic of a protease and a helicase. To examine the functions of JEV-NS3, a fusion protein of NS3 in Escherichia coli was generated. Analysis by Western blot using monospecific rabbit antisera generated against the fusion protein (anti-MBJEN3) showed that NS3 was localized in the membrane fraction of JEV-infected cells and the particulate fraction of bacteria extracts. The addition of anti-MBJEN3 sera reduced JEV-specific RNA synthesis activity in a in vitro system. In addition, NS3 was shown to exhibit RNA binding and ATPase activities, suggesting this protein has an important role in viral RNA replication in virus-infected cells.

Original languageEnglish (US)
Pages (from-to)105-112
Number of pages8
JournalVirus Genes
Volume9
Issue number2
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

Keywords

  • ATPase
  • Japanese encephalitis virus
  • RNA binding
  • expressed protein
  • nonstructural protein NS3

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

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