Late-phase detection of recent myocardial ischaemia using ultrasound molecular imaging targeted to intercellular adhesion molecule-1

Yi Yan, Yulin Liao, Li Yang, Juefei Wu, Jing Du, Wanling Xuan, Lijing Ji, Qiaobing Huang, Yili Liu, Jianping Bin

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Aims In this study, we attempted to detect a recent myocardial ischaemic event using ultrasound molecular imaging (UMI) with microbubbles (MB) targeted to intercellular adhesion molecule-1 (ICAM-1) in the late phase of reperfusion.Methods and results We created a myocardial ischaemiareperfusion model in 60 C57/BL male mice to simulate an angina attack (ischaemia for 15 min, reperfusion for 124 h). The degree of myocardial inflammation and levels of ICAM-1 protein were determined by histological and immunohistochemical analyses. UMI with MB targeted to endothelial ICAM-1, as well as routine non-invasive methods including electrocardiography, echocardiography, and plasma troponin I levels, were utilized to evaluate ischaemia over the time course of reperfusion. Levels of ICAM-1 in the vascular endothelium were significantly increased over the time course of reperfusion (824 h) of the ischaemic myocardium. The video intensity of ICAM-1 molecular images of the ischaemic anterior wall was almost three times greater than that in the non-ischaemic posterior wall during the late phase (824 h) of reperfusion. In contrast, routine methods yielded only weak evidence of ischaemia. Conclusion UMI with MB targeted to endothelial ICAM-1 provides reliable evidence of a recent myocardial ischaemic event in the late phase of reperfusion.

Original languageEnglish (US)
Pages (from-to)175-183
Number of pages9
JournalCardiovascular Research
Volume89
Issue number1
DOIs
StatePublished - Jan 1 2011
Externally publishedYes

Keywords

  • Contrast echocardiography
  • Intercellular adhesion molecule-1 (ICAM-1)
  • Microbubbles
  • Molecular imaging
  • Myocardial ischaemia

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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