Levels of α- and γ-tocopherol in human eyes

Evaluation of the possible role of IRBP in intraocular α-tocopherol transport

R. A. Alvarez, Gregory I Liou, S. L. Fong, C. D.B. Bridges

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Alpha-tocopherol was distributed almost equally between the retina and its underlying pigmented layers (pigment epithelium and choroid). Only 8.4% of the total α-tocopherol occurred in the iris and ciliary body. Alpha-tocopherol content was expressed as amount per eye, per cm2, and per 100 g wet weight. The combined retina and pigment epithelium choroid contained 2.9 ± 1.0 mg/100 g wet weight (χ̄ ± SD, n = 30 donors). Gamma-tocopherol represented 20.9 ± 12.2 mol % of the α-tocopherol. The anterior tissues contained 0.4 ± 0.2 mg/100 g (n = 19 donors). No significant correlation with age was found. Purified bovine interstitial retinol-binding protein (IRBP) bound exogenous 3H-α-tocopherol, which could be displaced by unlabeled all-trans retinol (K(D) = 10-6M). Much higher concentrations of unlabeled α-tocopherol were required to achieve a partial displacement of bound 3H-all-trans retinol. No endogenous α-tocopherol could be detected in bovine interphotoreceptor matrix.

Original languageEnglish (US)
Pages (from-to)481-487
Number of pages7
JournalAmerican Journal of Clinical Nutrition
Volume46
Issue number3
DOIs
StatePublished - Jan 1 1987

Fingerprint

Tocopherols
Choroid
alpha-Tocopherol
Vitamin A
Retina
Epithelium
gamma-Tocopherol
Weights and Measures
Ciliary Body
Iris
interstitial retinol-binding protein

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Levels of α- and γ-tocopherol in human eyes : Evaluation of the possible role of IRBP in intraocular α-tocopherol transport. / Alvarez, R. A.; Liou, Gregory I; Fong, S. L.; Bridges, C. D.B.

In: American Journal of Clinical Nutrition, Vol. 46, No. 3, 01.01.1987, p. 481-487.

Research output: Contribution to journalArticle

Alvarez, R. A. ; Liou, Gregory I ; Fong, S. L. ; Bridges, C. D.B. / Levels of α- and γ-tocopherol in human eyes : Evaluation of the possible role of IRBP in intraocular α-tocopherol transport. In: American Journal of Clinical Nutrition. 1987 ; Vol. 46, No. 3. pp. 481-487.
@article{48298238d6ae464a908ff80cb133b7f0,
title = "Levels of α- and γ-tocopherol in human eyes: Evaluation of the possible role of IRBP in intraocular α-tocopherol transport",
abstract = "Alpha-tocopherol was distributed almost equally between the retina and its underlying pigmented layers (pigment epithelium and choroid). Only 8.4{\%} of the total α-tocopherol occurred in the iris and ciliary body. Alpha-tocopherol content was expressed as amount per eye, per cm2, and per 100 g wet weight. The combined retina and pigment epithelium choroid contained 2.9 ± 1.0 mg/100 g wet weight (χ̄ ± SD, n = 30 donors). Gamma-tocopherol represented 20.9 ± 12.2 mol {\%} of the α-tocopherol. The anterior tissues contained 0.4 ± 0.2 mg/100 g (n = 19 donors). No significant correlation with age was found. Purified bovine interstitial retinol-binding protein (IRBP) bound exogenous 3H-α-tocopherol, which could be displaced by unlabeled all-trans retinol (K(D) = 10-6M). Much higher concentrations of unlabeled α-tocopherol were required to achieve a partial displacement of bound 3H-all-trans retinol. No endogenous α-tocopherol could be detected in bovine interphotoreceptor matrix.",
author = "Alvarez, {R. A.} and Liou, {Gregory I} and Fong, {S. L.} and Bridges, {C. D.B.}",
year = "1987",
month = "1",
day = "1",
doi = "10.1093/ajcn/46.3.481",
language = "English (US)",
volume = "46",
pages = "481--487",
journal = "American Journal of Clinical Nutrition",
issn = "0002-9165",
publisher = "American Society for Nutrition",
number = "3",

}

TY - JOUR

T1 - Levels of α- and γ-tocopherol in human eyes

T2 - Evaluation of the possible role of IRBP in intraocular α-tocopherol transport

AU - Alvarez, R. A.

AU - Liou, Gregory I

AU - Fong, S. L.

AU - Bridges, C. D.B.

PY - 1987/1/1

Y1 - 1987/1/1

N2 - Alpha-tocopherol was distributed almost equally between the retina and its underlying pigmented layers (pigment epithelium and choroid). Only 8.4% of the total α-tocopherol occurred in the iris and ciliary body. Alpha-tocopherol content was expressed as amount per eye, per cm2, and per 100 g wet weight. The combined retina and pigment epithelium choroid contained 2.9 ± 1.0 mg/100 g wet weight (χ̄ ± SD, n = 30 donors). Gamma-tocopherol represented 20.9 ± 12.2 mol % of the α-tocopherol. The anterior tissues contained 0.4 ± 0.2 mg/100 g (n = 19 donors). No significant correlation with age was found. Purified bovine interstitial retinol-binding protein (IRBP) bound exogenous 3H-α-tocopherol, which could be displaced by unlabeled all-trans retinol (K(D) = 10-6M). Much higher concentrations of unlabeled α-tocopherol were required to achieve a partial displacement of bound 3H-all-trans retinol. No endogenous α-tocopherol could be detected in bovine interphotoreceptor matrix.

AB - Alpha-tocopherol was distributed almost equally between the retina and its underlying pigmented layers (pigment epithelium and choroid). Only 8.4% of the total α-tocopherol occurred in the iris and ciliary body. Alpha-tocopherol content was expressed as amount per eye, per cm2, and per 100 g wet weight. The combined retina and pigment epithelium choroid contained 2.9 ± 1.0 mg/100 g wet weight (χ̄ ± SD, n = 30 donors). Gamma-tocopherol represented 20.9 ± 12.2 mol % of the α-tocopherol. The anterior tissues contained 0.4 ± 0.2 mg/100 g (n = 19 donors). No significant correlation with age was found. Purified bovine interstitial retinol-binding protein (IRBP) bound exogenous 3H-α-tocopherol, which could be displaced by unlabeled all-trans retinol (K(D) = 10-6M). Much higher concentrations of unlabeled α-tocopherol were required to achieve a partial displacement of bound 3H-all-trans retinol. No endogenous α-tocopherol could be detected in bovine interphotoreceptor matrix.

UR - http://www.scopus.com/inward/record.url?scp=0023514930&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023514930&partnerID=8YFLogxK

U2 - 10.1093/ajcn/46.3.481

DO - 10.1093/ajcn/46.3.481

M3 - Article

VL - 46

SP - 481

EP - 487

JO - American Journal of Clinical Nutrition

JF - American Journal of Clinical Nutrition

SN - 0002-9165

IS - 3

ER -