Levels of α- and γ-tocopherol in human eyes: Evaluation of the possible role of IRBP in intraocular α-tocopherol transport

R. A. Alvarez, G. I. Liou, S. L. Fong, C. D.B. Bridges

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Alpha-tocopherol was distributed almost equally between the retina and its underlying pigmented layers (pigment epithelium and choroid). Only 8.4% of the total α-tocopherol occurred in the iris and ciliary body. Alpha-tocopherol content was expressed as amount per eye, per cm2, and per 100 g wet weight. The combined retina and pigment epithelium choroid contained 2.9 ± 1.0 mg/100 g wet weight (χ̄ ± SD, n = 30 donors). Gamma-tocopherol represented 20.9 ± 12.2 mol % of the α-tocopherol. The anterior tissues contained 0.4 ± 0.2 mg/100 g (n = 19 donors). No significant correlation with age was found. Purified bovine interstitial retinol-binding protein (IRBP) bound exogenous 3H-α-tocopherol, which could be displaced by unlabeled all-trans retinol (K(D) = 10-6M). Much higher concentrations of unlabeled α-tocopherol were required to achieve a partial displacement of bound 3H-all-trans retinol. No endogenous α-tocopherol could be detected in bovine interphotoreceptor matrix.

Original languageEnglish (US)
Pages (from-to)481-487
Number of pages7
JournalAmerican Journal of Clinical Nutrition
Volume46
Issue number3
DOIs
StatePublished - Jan 1 1987

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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