Lifespan Is Prolonged in Autoimmune-Prone (NZB/NZW) F1 Mice Fed a Diet Supplemented with Indole-3-Carbinol

Karen J. Auborn, Mei Qi, Xiao J. Yan, Saul Teichberg, Da Zhi Chen, Michael P. Madaio, Nicholas Chiorazzi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Dietary modulation has the potential to prevent or ameliorate systemic lupus erythematosus (SLE). Indole-3-carbinol (I3C), which is abundant in cruciferous vegetables, was evaluated in a murine model of SLE because of its antiestrogenic activities. Female (NZB × NZW) F1 mice, which develop SLE, were fed an AIN76A diet without or with 0.2 g/kg I3C, starting soon after weaning or at 5 mo of age. At 12 mo of age, 80% of mice fed the I3C-supplemented diet soon after weaning were alive compared with only 10% of controls. When experimental diets were initiated at 5 mo of age, 100% of I3C fed mice and 30% of controls were alive at 12 mo of age. Anti-double-stranded DNA (dsDNA) antibodies developed in all groups, although at several time points, the levels produced in I3C-fed mice were significantly lower. Renal disease (proteinuria, histologic changes, IgG immune complex deposition, subepithelial deposits and diffuse epithelial cell foot process effacement) was more severe in controls with both protocols. The estrogen urinary metabolite ratio of 2- to 16α-hydroxyestrone was increased in I3C-fed mice. These findings demonstrate a profound effect of dietary I3C in experimental SLE.

Original languageEnglish (US)
Pages (from-to)3610-3613
Number of pages4
JournalJournal of Nutrition
Volume133
Issue number11
StatePublished - Nov 1 2003
Externally publishedYes

Fingerprint

Diet
Systemic Lupus Erythematosus
Weaning
indole-3-carbinol
Antigen-Antibody Complex
Proteinuria
Vegetables
Foot
Estrogens
Immunoglobulin G
Epithelial Cells
Kidney
Antibodies

Keywords

  • Anti-DNA antibodies
  • Autoimmunity
  • Glomerulonephritis
  • Indole-3-carbinol
  • Systemic lupus erythematosus

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Auborn, K. J., Qi, M., Yan, X. J., Teichberg, S., Chen, D. Z., Madaio, M. P., & Chiorazzi, N. (2003). Lifespan Is Prolonged in Autoimmune-Prone (NZB/NZW) F1 Mice Fed a Diet Supplemented with Indole-3-Carbinol. Journal of Nutrition, 133(11), 3610-3613.

Lifespan Is Prolonged in Autoimmune-Prone (NZB/NZW) F1 Mice Fed a Diet Supplemented with Indole-3-Carbinol. / Auborn, Karen J.; Qi, Mei; Yan, Xiao J.; Teichberg, Saul; Chen, Da Zhi; Madaio, Michael P.; Chiorazzi, Nicholas.

In: Journal of Nutrition, Vol. 133, No. 11, 01.11.2003, p. 3610-3613.

Research output: Contribution to journalArticle

Auborn, KJ, Qi, M, Yan, XJ, Teichberg, S, Chen, DZ, Madaio, MP & Chiorazzi, N 2003, 'Lifespan Is Prolonged in Autoimmune-Prone (NZB/NZW) F1 Mice Fed a Diet Supplemented with Indole-3-Carbinol', Journal of Nutrition, vol. 133, no. 11, pp. 3610-3613.
Auborn KJ, Qi M, Yan XJ, Teichberg S, Chen DZ, Madaio MP et al. Lifespan Is Prolonged in Autoimmune-Prone (NZB/NZW) F1 Mice Fed a Diet Supplemented with Indole-3-Carbinol. Journal of Nutrition. 2003 Nov 1;133(11):3610-3613.
Auborn, Karen J. ; Qi, Mei ; Yan, Xiao J. ; Teichberg, Saul ; Chen, Da Zhi ; Madaio, Michael P. ; Chiorazzi, Nicholas. / Lifespan Is Prolonged in Autoimmune-Prone (NZB/NZW) F1 Mice Fed a Diet Supplemented with Indole-3-Carbinol. In: Journal of Nutrition. 2003 ; Vol. 133, No. 11. pp. 3610-3613.
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abstract = "Dietary modulation has the potential to prevent or ameliorate systemic lupus erythematosus (SLE). Indole-3-carbinol (I3C), which is abundant in cruciferous vegetables, was evaluated in a murine model of SLE because of its antiestrogenic activities. Female (NZB × NZW) F1 mice, which develop SLE, were fed an AIN76A diet without or with 0.2 g/kg I3C, starting soon after weaning or at 5 mo of age. At 12 mo of age, 80{\%} of mice fed the I3C-supplemented diet soon after weaning were alive compared with only 10{\%} of controls. When experimental diets were initiated at 5 mo of age, 100{\%} of I3C fed mice and 30{\%} of controls were alive at 12 mo of age. Anti-double-stranded DNA (dsDNA) antibodies developed in all groups, although at several time points, the levels produced in I3C-fed mice were significantly lower. Renal disease (proteinuria, histologic changes, IgG immune complex deposition, subepithelial deposits and diffuse epithelial cell foot process effacement) was more severe in controls with both protocols. The estrogen urinary metabolite ratio of 2- to 16α-hydroxyestrone was increased in I3C-fed mice. These findings demonstrate a profound effect of dietary I3C in experimental SLE.",
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