Lipid peroxidation associated cardiolipin loss and membrane depolarization in rat brain mitochondria

Tanusree Sen, Nilkantha Sen, Gayatri Tripathi, Uttara Chatterjee, Sasanka Chakrabarti

Research output: Contribution to journalArticle

63 Scopus citations

Abstract

Oxidative stress induced by Fe2+ (50 μM) and ascorbate (2 mM) in isolated rat brain mitochondria incubated in vitro leads to an enhanced lipid peroxidation, cardiolipin loss and an increased formation of protein carbonyls. These changes are associated with a loss of mitochondrial membrane potential (depolarization) and an impaired activity of electron transport chain (ETC) as measured by MTT reduction assay. Butylated hydroxytoluene (0.2 mM), an inhibitor of lipid peroxidation, can prevent significantly the loss of cardiolipin, the increased protein carbonyl formation and the decrease in mitochondrial membrane potential induced by Fe2+ and ascorbate, implying that the changes are secondary to membrane lipid peroxidation. However, iron-ascorbate induced impairment of mitochondrial ETC activity is apparently independent of lipid peroxidation process. The structural and functional derangement of mitochondria induced by oxidative stress as reported here may have implications in neuronal damage associated with brain aging and neurodegenerative disorders.

Original languageEnglish (US)
Pages (from-to)20-27
Number of pages8
JournalNeurochemistry International
Volume49
Issue number1
DOIs
StatePublished - Jul 1 2006

Keywords

  • Cardiolipin
  • Electron transport chain
  • Lipid peroxidation
  • Mitochondria
  • Mitochondrial membrane potential
  • Reactive oxygen species

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

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