TY - JOUR
T1 - Localization and immunological characterization of antigenic domains of the rabies virus internal N and NS proteins
AU - Dietzschold, Bernhard
AU - Lafon, Monique
AU - Wang, Honghai
AU - Otvos, Laszlo
AU - Celis, Esteban
AU - Wunner, William H.
AU - Koprowski, Hilary
N1 - Funding Information:
We wish to thank the National Institute of Allergy and Infectious Diseases for their generous support by grants AI-09706 and AI-18883. We thank James Earley, Thomas Gay and Georgia Krivulka for their excellent technical assistance, and we are grateful to Marina Hoffman (editor) for help in preparing this manuscript.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1987/8
Y1 - 1987/8
N2 - To locate epitopes on internal antigens of rabies virus, purified N and NS proteins of the nucleocapsid were cleaved at methionine, tryptophan or glutamic acid residues, transferred to nitrocellulose and immunostained using monoclonal antibodies (MAbs) specific for N and NS proteins, respectively. Five MAb-positive fragments of N protein and one fragment of NS protein were located after NH2-terminal amino acid sequence analysis within the deduced amino acid sequences of N and NS proteins. Antigenic analysis of synthetic overlapping peptides corresponding to the amino acid sequences of these fragments localized two major antigenic sites of N protein and one antigenic site of NS protein. Like the N- and NS-specific MAbs, anti-peptide antisera produced against the different synthetic antigens either reacted in a type-common fashion with all rabies virus strains, or in a type-specific manner with a restricted number of strains. The synthetic peptides corresponding to the three antigenic regions of the N and NS proteins also stimulated proliferation of human T lymphocytes derived from vaccinees who received inactivated rabies virus vaccine. This suggested that the antigenic regions of N and NS proteins are recognized by both B and T cells.
AB - To locate epitopes on internal antigens of rabies virus, purified N and NS proteins of the nucleocapsid were cleaved at methionine, tryptophan or glutamic acid residues, transferred to nitrocellulose and immunostained using monoclonal antibodies (MAbs) specific for N and NS proteins, respectively. Five MAb-positive fragments of N protein and one fragment of NS protein were located after NH2-terminal amino acid sequence analysis within the deduced amino acid sequences of N and NS proteins. Antigenic analysis of synthetic overlapping peptides corresponding to the amino acid sequences of these fragments localized two major antigenic sites of N protein and one antigenic site of NS protein. Like the N- and NS-specific MAbs, anti-peptide antisera produced against the different synthetic antigens either reacted in a type-common fashion with all rabies virus strains, or in a type-specific manner with a restricted number of strains. The synthetic peptides corresponding to the three antigenic regions of the N and NS proteins also stimulated proliferation of human T lymphocytes derived from vaccinees who received inactivated rabies virus vaccine. This suggested that the antigenic regions of N and NS proteins are recognized by both B and T cells.
KW - B cell determinant
KW - Phosphorylation site
KW - Rabies virus nucleocapsid
KW - T cell determinant
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U2 - 10.1016/0168-1702(87)90023-2
DO - 10.1016/0168-1702(87)90023-2
M3 - Article
C2 - 2445121
AN - SCOPUS:0023391546
SN - 0168-1702
VL - 8
SP - 103
EP - 125
JO - Virus Research
JF - Virus Research
IS - 2
ER -