Localization of the 8;13 translocation breakpoint associated with myeloproliferative disease to a 1.5 mbp region of chromosome 13

Helena Kempski, Donald Macdonald, Antony J. Michalski, Terry Roberts, John M. Goldman, Nicholas C.P. Cross, John Kenneth Cowell

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

There are five reported cases of an atypical myeloproliferative disorder in which the leukemia cells have a consistent t(8;13)(p11;q12) translocation. We analyzed the breakpoint in metaphases from two of these patients by fluorescence in situ hybridization using a series of yeast artificial chromosomes (YACs) derived from the 13q12 region. We found that a YAC containing the FLT1 and FLT3 oncogenes was localized distal to the 13q12 breakpoint and was not rearranged. YAC 66, a YAC that lies immediately adjacent to the chromosome 13 centromere, was localized proximal to the 13q12 breakpoint and was not rearranged. A third YAC, which is located between FLT1 and YAC 66, was unrearranged in normal metaphase chromosomes, but showed hybridization signals on both derivative chromosomes in both cases. Thus, the breakpoints in these two cases are localized to the same 1.5 Mbp region of 13q12. This may be the site of an unidentified gene involved in the pathogenesis of some types of leukemia.

Original languageEnglish (US)
Pages (from-to)283-287
Number of pages5
JournalGenes, Chromosomes and Cancer
Volume12
Issue number4
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

Yeast Artificial Chromosomes
Chromosomes, Human, Pair 13
Metaphase
Leukemia
Chromosomes
Myeloproliferative Disorders
Centromere
Fluorescence In Situ Hybridization
Oncogenes
Genes

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

Cite this

Localization of the 8;13 translocation breakpoint associated with myeloproliferative disease to a 1.5 mbp region of chromosome 13. / Kempski, Helena; Macdonald, Donald; Michalski, Antony J.; Roberts, Terry; Goldman, John M.; Cross, Nicholas C.P.; Cowell, John Kenneth.

In: Genes, Chromosomes and Cancer, Vol. 12, No. 4, 01.01.1995, p. 283-287.

Research output: Contribution to journalArticle

Kempski, Helena ; Macdonald, Donald ; Michalski, Antony J. ; Roberts, Terry ; Goldman, John M. ; Cross, Nicholas C.P. ; Cowell, John Kenneth. / Localization of the 8;13 translocation breakpoint associated with myeloproliferative disease to a 1.5 mbp region of chromosome 13. In: Genes, Chromosomes and Cancer. 1995 ; Vol. 12, No. 4. pp. 283-287.
@article{a8a6c02ef0c44da59d32de899d1d6494,
title = "Localization of the 8;13 translocation breakpoint associated with myeloproliferative disease to a 1.5 mbp region of chromosome 13",
abstract = "There are five reported cases of an atypical myeloproliferative disorder in which the leukemia cells have a consistent t(8;13)(p11;q12) translocation. We analyzed the breakpoint in metaphases from two of these patients by fluorescence in situ hybridization using a series of yeast artificial chromosomes (YACs) derived from the 13q12 region. We found that a YAC containing the FLT1 and FLT3 oncogenes was localized distal to the 13q12 breakpoint and was not rearranged. YAC 66, a YAC that lies immediately adjacent to the chromosome 13 centromere, was localized proximal to the 13q12 breakpoint and was not rearranged. A third YAC, which is located between FLT1 and YAC 66, was unrearranged in normal metaphase chromosomes, but showed hybridization signals on both derivative chromosomes in both cases. Thus, the breakpoints in these two cases are localized to the same 1.5 Mbp region of 13q12. This may be the site of an unidentified gene involved in the pathogenesis of some types of leukemia.",
author = "Helena Kempski and Donald Macdonald and Michalski, {Antony J.} and Terry Roberts and Goldman, {John M.} and Cross, {Nicholas C.P.} and Cowell, {John Kenneth}",
year = "1995",
month = "1",
day = "1",
doi = "10.1002/gcc.2870120408",
language = "English (US)",
volume = "12",
pages = "283--287",
journal = "Genes Chromosomes and Cancer",
issn = "1045-2257",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - Localization of the 8;13 translocation breakpoint associated with myeloproliferative disease to a 1.5 mbp region of chromosome 13

AU - Kempski, Helena

AU - Macdonald, Donald

AU - Michalski, Antony J.

AU - Roberts, Terry

AU - Goldman, John M.

AU - Cross, Nicholas C.P.

AU - Cowell, John Kenneth

PY - 1995/1/1

Y1 - 1995/1/1

N2 - There are five reported cases of an atypical myeloproliferative disorder in which the leukemia cells have a consistent t(8;13)(p11;q12) translocation. We analyzed the breakpoint in metaphases from two of these patients by fluorescence in situ hybridization using a series of yeast artificial chromosomes (YACs) derived from the 13q12 region. We found that a YAC containing the FLT1 and FLT3 oncogenes was localized distal to the 13q12 breakpoint and was not rearranged. YAC 66, a YAC that lies immediately adjacent to the chromosome 13 centromere, was localized proximal to the 13q12 breakpoint and was not rearranged. A third YAC, which is located between FLT1 and YAC 66, was unrearranged in normal metaphase chromosomes, but showed hybridization signals on both derivative chromosomes in both cases. Thus, the breakpoints in these two cases are localized to the same 1.5 Mbp region of 13q12. This may be the site of an unidentified gene involved in the pathogenesis of some types of leukemia.

AB - There are five reported cases of an atypical myeloproliferative disorder in which the leukemia cells have a consistent t(8;13)(p11;q12) translocation. We analyzed the breakpoint in metaphases from two of these patients by fluorescence in situ hybridization using a series of yeast artificial chromosomes (YACs) derived from the 13q12 region. We found that a YAC containing the FLT1 and FLT3 oncogenes was localized distal to the 13q12 breakpoint and was not rearranged. YAC 66, a YAC that lies immediately adjacent to the chromosome 13 centromere, was localized proximal to the 13q12 breakpoint and was not rearranged. A third YAC, which is located between FLT1 and YAC 66, was unrearranged in normal metaphase chromosomes, but showed hybridization signals on both derivative chromosomes in both cases. Thus, the breakpoints in these two cases are localized to the same 1.5 Mbp region of 13q12. This may be the site of an unidentified gene involved in the pathogenesis of some types of leukemia.

UR - http://www.scopus.com/inward/record.url?scp=0028946702&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028946702&partnerID=8YFLogxK

U2 - 10.1002/gcc.2870120408

DO - 10.1002/gcc.2870120408

M3 - Article

VL - 12

SP - 283

EP - 287

JO - Genes Chromosomes and Cancer

JF - Genes Chromosomes and Cancer

SN - 1045-2257

IS - 4

ER -