Loss of DP1 Aggravates Vascular Remodeling in Pulmonary Arterial Hypertension via mTORC1 Signaling

Yuhu He, Caojian Zuo, Daile Jia, Peiyuan Bai, Deping Kong, Di Chen, Guizhu Liu, Juanjuan Li, Yuanyang Wang, Guilin Chen, Shuai Yan, Bing Xiao, Jian Zhang, Lingjuan Piao, Yanli Li, Yi Deng, Bin Li, Philippe P. Roux, Katrin I. Andreasson, Richard M. BreyerYunchao Su, Jian Wang, Ankang Lyu, Yujun Shen, Ying Yu

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Rationale: Vascular remodeling, including smooth muscle cell hypertrophy and proliferation, is the key pathological feature of pulmonary arterial hypertension (PAH). Prostaglandin I2 analogs (beraprost, iloprost, and treprostinil) are effective in the treatment of PAH. Of note, the clinically favorable effects of treprostinil in severe PAH may be attributable to concomitant activation of DP1 (D prostanoid receptor subtype 1).Objectives: To study the role of DP1 in the progression of PAH and its underlying mechanism.Methods: DP1 levels were examined in pulmonary arteries of patients and animals with PAH. Multiple genetic and pharmacologic approaches were used to investigate DP1-mediated signaling in PAH.Measurements and Main Results: DP1 expression was downregulated in hypoxia-treated pulmonary artery smooth muscle cells and in pulmonary arteries from rodent PAH models and patients with idiopathic PAH. DP1 deletion exacerbated pulmonary artery remodeling in hypoxia-induced PAH, whereas pharmacological activation or forced expression of the DP1 receptor had the opposite effect in different rodent models. DP1 deficiency promoted pulmonary artery smooth muscle cell hypertrophy and proliferation in response to hypoxia via induction of mTORC1 (mammalian target of rapamycin complex 1) activity. Rapamycin, an inhibitor of mTORC1, alleviated the hypoxia-induced exacerbation of PAH in DP1-knockout mice. DP1 activation facilitated raptor dissociation from mTORC1 and suppressed mTORC1 activity through PKA (protein kinase A)-dependent phosphorylation of raptor at Ser791. Moreover, treprostinil treatment blocked the progression of hypoxia-induced PAH in mice in part by targeting the DP1 receptor.Conclusions: DP1 activation attenuates hypoxia-induced pulmonary artery remodeling and PAH through PKA-mediated dissociation of raptor from mTORC1. These results suggest that the DP1 receptor may serve as a therapeutic target for the management of PAH.

Original languageEnglish (US)
Pages (from-to)1263-1276
Number of pages14
JournalAmerican journal of respiratory and critical care medicine
Volume201
Issue number10
DOIs
StatePublished - May 15 2020

Keywords

  • DP1 receptor
  • hypertrophy and proliferation
  • mTOR signaling
  • pulmonary arterial hypertension
  • pulmonary artery smooth muscle cell

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

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    He, Y., Zuo, C., Jia, D., Bai, P., Kong, D., Chen, D., Liu, G., Li, J., Wang, Y., Chen, G., Yan, S., Xiao, B., Zhang, J., Piao, L., Li, Y., Deng, Y., Li, B., Roux, P. P., Andreasson, K. I., ... Yu, Y. (2020). Loss of DP1 Aggravates Vascular Remodeling in Pulmonary Arterial Hypertension via mTORC1 Signaling. American journal of respiratory and critical care medicine, 201(10), 1263-1276. https://doi.org/10.1164/rccm.201911-2137OC