Mammalian-cell-produced neurturin (NTN) is more potent than purified Escherichia coli-produced NTN

Michael R. Hoane, Kamal D. Puri, Lei Xu, Paul F. Stabila, Hongmei Zhao, Amit G. Gulwadi, Heidi S. Phillips, Brigitte Devaux, Mark D. Lindner, Weng Tao

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Neurturin (NTN) is a recently identified homologue of glial-cell-line- derived neurotrophic factor. Both factors promote the survival of dopaminergic (DA) neurons. We investigated the biological activity of mammalian-cell-produced NTN versus purified Escherichia coli-produced NTN. Baby hamster kidney cells were engineered to stably secrete mature human NTN. Mammalian-cell-derived NTN enhanced the activity of embryonic DA neurons in vitro, with greater potency (maximum effect achieved in the picogram range) than purified E. coli-produced NTN. Cell-based delivery of NTN (less than 10 ng/day) was also shown to be biologically active in vivo. These results suggest that mammalian-cell-derived NTN, synthesized de novo and delivered in small quantities to the parenchyma at the target site, may be as active as much larger quantities of purified, E. coli-produced NTN, delivered by other means. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)189-193
Number of pages5
JournalExperimental Neurology
Volume162
Issue number1
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

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Neurturin
Escherichia coli
Dopaminergic Neurons
Glial Cell Line-Derived Neurotrophic Factor
Cricetinae

Keywords

  • Bioactivity
  • Delivery
  • Neurturin

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

Cite this

Mammalian-cell-produced neurturin (NTN) is more potent than purified Escherichia coli-produced NTN. / Hoane, Michael R.; Puri, Kamal D.; Xu, Lei; Stabila, Paul F.; Zhao, Hongmei; Gulwadi, Amit G.; Phillips, Heidi S.; Devaux, Brigitte; Lindner, Mark D.; Tao, Weng.

In: Experimental Neurology, Vol. 162, No. 1, 01.01.2000, p. 189-193.

Research output: Contribution to journalArticle

Hoane, MR, Puri, KD, Xu, L, Stabila, PF, Zhao, H, Gulwadi, AG, Phillips, HS, Devaux, B, Lindner, MD & Tao, W 2000, 'Mammalian-cell-produced neurturin (NTN) is more potent than purified Escherichia coli-produced NTN', Experimental Neurology, vol. 162, no. 1, pp. 189-193. https://doi.org/10.1006/exnr.2000.7311
Hoane, Michael R. ; Puri, Kamal D. ; Xu, Lei ; Stabila, Paul F. ; Zhao, Hongmei ; Gulwadi, Amit G. ; Phillips, Heidi S. ; Devaux, Brigitte ; Lindner, Mark D. ; Tao, Weng. / Mammalian-cell-produced neurturin (NTN) is more potent than purified Escherichia coli-produced NTN. In: Experimental Neurology. 2000 ; Vol. 162, No. 1. pp. 189-193.
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