Marginal zone B cells exhibit unique activation, proliferative and immunoglobulin secretory responses

Alyce M. Oliver, Flavius Martin, G. Larry Gartland, Robert H. Carter, John F. Kearney

Research output: Contribution to journalArticle

329 Citations (Scopus)

Abstract

Mouse splenic B cells can be separated based on their distinctive expression of cell surface antigens. Marginal zone (MZ) B cells are CD38(high) CD21(high) CD23(low/-), while follicular (FO) B cells are CD21(int) CD23(int) and newly formed (NF) B cells are CD21(dim/-) CD23-. Exploiting these phenotypic distinctions, we isolated the three B cell subsets and assessed their other phenotypic differences and functional capabilities in vitro. FO B cells proliferate more than the other B cell subsets in response to either IgM or CD38 cross-linking. MZ B cells proliferate better than FO B cells when stimulated with lipopolysaccharide (LPS), sub-optimal levels of LPS and CD38 cross-linking or CD40 ligation. When NF, FO and MZ B cells were stimulated with either LPS or LPS and interleukin-4, MZ B cells secreted more IgM and IgG3 than the other two subsets. Similarly, calcium fluxes measured within MZ B cells are larger in amplitude and more sustained after B cell receptor cross-linking than those found in the other two subsets. Collectively, these results indicate that CD38(high) CD21(high) CD23(low/-) MZ B cells are specially suited to play a unique role in response to antigens delivered to the MZ area.

Original languageEnglish (US)
Pages (from-to)2366-2374
Number of pages9
JournalEuropean Journal of Immunology
Volume27
Issue number9
DOIs
StatePublished - Sep 1 1997

Fingerprint

Immunoglobulins
B-Lymphocytes
Lipopolysaccharides
B-Lymphocyte Subsets
Immunoglobulin M
Surface Antigens
Interleukin-4
Ligation
Immunoglobulin G
Calcium
Antigens

Keywords

  • B cell
  • CD21
  • CD38
  • Marginal zone
  • Mature B cell subsets

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Marginal zone B cells exhibit unique activation, proliferative and immunoglobulin secretory responses. / Oliver, Alyce M.; Martin, Flavius; Gartland, G. Larry; Carter, Robert H.; Kearney, John F.

In: European Journal of Immunology, Vol. 27, No. 9, 01.09.1997, p. 2366-2374.

Research output: Contribution to journalArticle

Oliver, Alyce M. ; Martin, Flavius ; Gartland, G. Larry ; Carter, Robert H. ; Kearney, John F. / Marginal zone B cells exhibit unique activation, proliferative and immunoglobulin secretory responses. In: European Journal of Immunology. 1997 ; Vol. 27, No. 9. pp. 2366-2374.
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