Maternal group B Streptococcus and the infant gut microbiota

A. E. Cassidy-Bushrow, A. Sitarik, A. M. Levin, S. V. Lynch, S. Havstad, Dennis Randall Ownby, C. C. Johnson, G. Wegienka

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Early patterns of gut colonization may predispose children to adult disease. Exposures in utero and during delivery are associated with the infant gut microbiome. Although ~35% of women carry group B strep (GBS; Streptococcus agalactiae) during pregnancy, it is unknown if GBS presence influences the infant gut microbiome. As part of a population-based, general risk birth cohort, stool specimens were collected from infant's diapers at research visits conducted at ~1 and 6 months of age. Using the Illumina MiSeq (San Diego, CA) platform, the V4 region of the bacterial 16S rRNA gene was sequenced. Infant gut bacterial community compositional differences by maternal GBS status were evaluated using permutational multivariate analysis of variance. Individual operational taxonomic units (OTUs) were tested using a zero-inflated negative binomial model. Data on maternal GBS and infant gut microbiota from either 1 (n=112) or 6-month-old stool (n=150) specimens was available on 262 maternal-child pairs. Eighty women (30.5%) were GBS+, of who 58 (72.5%) were given intrapartum antibiotics. After adjusting for maternal race, prenatal antifungal use and intrapartum antibiotics, maternal GBS status was statistically significantly associated with gut bacterial composition in the 6 month visit specimen (Canberra R2=0.008, P=0.008; Unweighted UniFrac R2=0.010, P=0.011). Individual OTU tests revealed that infants of GBS+ mothers were significantly enriched for specific members of the Clostridiaceae, Ruminococcoceae, and Enterococcaceae in the 6 month specimens compared with infants of GBS- mothers. Whether these taxonomic differences in infant gut microbiota at 6 months lead to differential predisposition for adult disease requires additional study.

Original languageEnglish (US)
Pages (from-to)45-53
Number of pages9
JournalJournal of Developmental Origins of Health and Disease
Volume7
Issue number1
DOIs
StatePublished - Jan 1 2016

Fingerprint

Streptococcus agalactiae
Mothers
Infant Diapers
Enterococcaceae
Anti-Bacterial Agents
Statistical Models
Gastrointestinal Microbiome
rRNA Genes
Analysis of Variance
Multivariate Analysis
Parturition
Pregnancy
Research
Population

Keywords

  • antibiotics
  • developmental origins
  • group B strep
  • gut microbiota

ASJC Scopus subject areas

  • Medicine (miscellaneous)

Cite this

Cassidy-Bushrow, A. E., Sitarik, A., Levin, A. M., Lynch, S. V., Havstad, S., Ownby, D. R., ... Wegienka, G. (2016). Maternal group B Streptococcus and the infant gut microbiota. Journal of Developmental Origins of Health and Disease, 7(1), 45-53. https://doi.org/10.1017/S2040174415001361

Maternal group B Streptococcus and the infant gut microbiota. / Cassidy-Bushrow, A. E.; Sitarik, A.; Levin, A. M.; Lynch, S. V.; Havstad, S.; Ownby, Dennis Randall; Johnson, C. C.; Wegienka, G.

In: Journal of Developmental Origins of Health and Disease, Vol. 7, No. 1, 01.01.2016, p. 45-53.

Research output: Contribution to journalArticle

Cassidy-Bushrow, AE, Sitarik, A, Levin, AM, Lynch, SV, Havstad, S, Ownby, DR, Johnson, CC & Wegienka, G 2016, 'Maternal group B Streptococcus and the infant gut microbiota', Journal of Developmental Origins of Health and Disease, vol. 7, no. 1, pp. 45-53. https://doi.org/10.1017/S2040174415001361
Cassidy-Bushrow, A. E. ; Sitarik, A. ; Levin, A. M. ; Lynch, S. V. ; Havstad, S. ; Ownby, Dennis Randall ; Johnson, C. C. ; Wegienka, G. / Maternal group B Streptococcus and the infant gut microbiota. In: Journal of Developmental Origins of Health and Disease. 2016 ; Vol. 7, No. 1. pp. 45-53.
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