Measurement of the Membrane Potential of Isolated Nerve Terminals by the Lipophilic Cation [3H]Triphenylmethylphosphonium Bromide

Elisabeth Hansson, Ingemar Jacobson, Richard C Venema, Åke Sellström

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Abstract: The lipophilic cation [3H]triphenylrnethylphosphonium bromide ([3H]TPMP+) was investigated as a measure of the membrane potential of synaptosomes. Conditions under which [3H]TPMP+ achieved an equilibrium distribution were tested. The toxicity of TPMP has been studied relative to its inhibitory effects on [3H]y‐aminobutyric acid ([3H]GABA) transport. In some experiments the distribution of 86RbZ+ and [3H]TPMP+ was changed upon incubation in the presence of elevated levels of K+, ouabain, or KCN, or at 0°C in a way that would be expected from the membrane potential. In normal incubation conditions a membrane potential of ∼−60 mv was calculated.

Original languageEnglish (US)
Pages (from-to)569-573
Number of pages5
JournalJournal of Neurochemistry
Volume34
Issue number3
DOIs
StatePublished - Jan 1 1980

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Membrane Potentials
Cations
Membranes
Synaptosomes
Ouabain
Bromides
gamma-Aminobutyric Acid
Toxicity
Acids
triphenylmethylphosphonium
Experiments

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Measurement of the Membrane Potential of Isolated Nerve Terminals by the Lipophilic Cation [3H]Triphenylmethylphosphonium Bromide. / Hansson, Elisabeth; Jacobson, Ingemar; Venema, Richard C; Sellström, Åke.

In: Journal of Neurochemistry, Vol. 34, No. 3, 01.01.1980, p. 569-573.

Research output: Contribution to journalArticle

Hansson, Elisabeth ; Jacobson, Ingemar ; Venema, Richard C ; Sellström, Åke. / Measurement of the Membrane Potential of Isolated Nerve Terminals by the Lipophilic Cation [3H]Triphenylmethylphosphonium Bromide. In: Journal of Neurochemistry. 1980 ; Vol. 34, No. 3. pp. 569-573.
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