Mechanical Disruption of Dentin Collagen Fibrils during Resin-Dentin Bond Testing

Franklin Chi Meng Tay, Ricardo M. Carvalho, C. K.Y. Yiu, N. M. King, Y. Zhang, K. Agee, S. Bouillaguet, David Henry Pashley

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Purpose: To determine if collagen fibrils on the dentin side of failed resin-dentin interfaces undergo mechanical disruption during microtensile bond testing. Materials and Methods: Extracted, caries-free human third molars were divided into four groups. The occlusal enamel was removed, leaving a flat dentin surface for bonding. Resin composite buildups were made after the acid-conditioned dentin was bonded with either Single Bond (S) or One-Step (0), and using either moist bonding (M) or air drying for 5 s (D). After storage in water for 24 h, the teeth were vertically sectioned into an array of 0.9 x 0.9 mm resin composite-dentin beams. They were stressed to failure using the nontrimming version of the microtensile bond test. Fractured dentin and resin composite sides of representative beams from each group that exhibited adhesive failures under light microscopy examination were prepared for scanning (SEM) and transmission electron microscopy (TEM). Results: A two-way ANOVA showed that moist bond strengths were significantly higher than those made to dry dentin (M > D; p < 0.001), but that there was no difference between the adhesives (S vs 0; p = 0.547). SEM analysis showed the presence of loose collagen fibrils within fractured hybrid layers in the dry groups, but not in the moist groups. TEM examination of the dry-bonded groups revealed collagen fibrils that were thinner and exhibited abnormally wide interfibrillar spaces when hybrid layers were intact. Within dry-bonded fractured hybrid layers, broad mechanical disruption zones could be seen, consisting of fibrils that were devoid of cross banding, defibrillation of the subfibrillar architecture, and gross disaggregation into microfibrils. In the moist-bonded groups, only short mechanical disruption zones were found along the torn edges of the collagen fibrils. The rest of the fibrils beyond the fracture site were intact and retained their periodicity. Mechanical testing of demineralized matrices yielded a maximum modulus of elasticity of 43.9 ±6.1 MPa. Conclusion: We speculate that adhesive resin has a protective function for demineralized collagen in wellinfiltrated hybrid layers. We propose that both the collagen and resin contribute to load sharing during stress application until the final moment of rupture. On the other hand, collagen fibrils in poorly infiltrated hybrid layers, being unsupported by resin, undergo various degrees of irreversible mechanical disruption depending on how the stress is dissipated. The collagen fibril network has a much lower modulus of elasticity compared to those of resin-infiltrated fibrils or the demineralized dentin.

Original languageEnglish (US)
Pages (from-to)175-192
Number of pages18
JournalJournal of Adhesive Dentistry
Volume2
Issue number3
StatePublished - Dec 1 2000

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Dentin
Collagen
Composite Resins
Adhesives
Elastic Modulus
Microfibrils
Scanning Transmission Electron Microscopy
Third Molar
Periodicity
Dental Enamel
Transmission Electron Microscopy
Rupture
Microscopy
Analysis of Variance
Tooth
Air
Light
Acids
Water

ASJC Scopus subject areas

  • Orthodontics
  • Oral Surgery
  • Periodontics

Cite this

Tay, F. C. M., Carvalho, R. M., Yiu, C. K. Y., King, N. M., Zhang, Y., Agee, K., ... Pashley, D. H. (2000). Mechanical Disruption of Dentin Collagen Fibrils during Resin-Dentin Bond Testing. Journal of Adhesive Dentistry, 2(3), 175-192.

Mechanical Disruption of Dentin Collagen Fibrils during Resin-Dentin Bond Testing. / Tay, Franklin Chi Meng; Carvalho, Ricardo M.; Yiu, C. K.Y.; King, N. M.; Zhang, Y.; Agee, K.; Bouillaguet, S.; Pashley, David Henry.

In: Journal of Adhesive Dentistry, Vol. 2, No. 3, 01.12.2000, p. 175-192.

Research output: Contribution to journalArticle

Tay, FCM, Carvalho, RM, Yiu, CKY, King, NM, Zhang, Y, Agee, K, Bouillaguet, S & Pashley, DH 2000, 'Mechanical Disruption of Dentin Collagen Fibrils during Resin-Dentin Bond Testing', Journal of Adhesive Dentistry, vol. 2, no. 3, pp. 175-192.
Tay FCM, Carvalho RM, Yiu CKY, King NM, Zhang Y, Agee K et al. Mechanical Disruption of Dentin Collagen Fibrils during Resin-Dentin Bond Testing. Journal of Adhesive Dentistry. 2000 Dec 1;2(3):175-192.
Tay, Franklin Chi Meng ; Carvalho, Ricardo M. ; Yiu, C. K.Y. ; King, N. M. ; Zhang, Y. ; Agee, K. ; Bouillaguet, S. ; Pashley, David Henry. / Mechanical Disruption of Dentin Collagen Fibrils during Resin-Dentin Bond Testing. In: Journal of Adhesive Dentistry. 2000 ; Vol. 2, No. 3. pp. 175-192.
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N2 - Purpose: To determine if collagen fibrils on the dentin side of failed resin-dentin interfaces undergo mechanical disruption during microtensile bond testing. Materials and Methods: Extracted, caries-free human third molars were divided into four groups. The occlusal enamel was removed, leaving a flat dentin surface for bonding. Resin composite buildups were made after the acid-conditioned dentin was bonded with either Single Bond (S) or One-Step (0), and using either moist bonding (M) or air drying for 5 s (D). After storage in water for 24 h, the teeth were vertically sectioned into an array of 0.9 x 0.9 mm resin composite-dentin beams. They were stressed to failure using the nontrimming version of the microtensile bond test. Fractured dentin and resin composite sides of representative beams from each group that exhibited adhesive failures under light microscopy examination were prepared for scanning (SEM) and transmission electron microscopy (TEM). Results: A two-way ANOVA showed that moist bond strengths were significantly higher than those made to dry dentin (M > D; p < 0.001), but that there was no difference between the adhesives (S vs 0; p = 0.547). SEM analysis showed the presence of loose collagen fibrils within fractured hybrid layers in the dry groups, but not in the moist groups. TEM examination of the dry-bonded groups revealed collagen fibrils that were thinner and exhibited abnormally wide interfibrillar spaces when hybrid layers were intact. Within dry-bonded fractured hybrid layers, broad mechanical disruption zones could be seen, consisting of fibrils that were devoid of cross banding, defibrillation of the subfibrillar architecture, and gross disaggregation into microfibrils. In the moist-bonded groups, only short mechanical disruption zones were found along the torn edges of the collagen fibrils. The rest of the fibrils beyond the fracture site were intact and retained their periodicity. Mechanical testing of demineralized matrices yielded a maximum modulus of elasticity of 43.9 ±6.1 MPa. Conclusion: We speculate that adhesive resin has a protective function for demineralized collagen in wellinfiltrated hybrid layers. We propose that both the collagen and resin contribute to load sharing during stress application until the final moment of rupture. On the other hand, collagen fibrils in poorly infiltrated hybrid layers, being unsupported by resin, undergo various degrees of irreversible mechanical disruption depending on how the stress is dissipated. The collagen fibril network has a much lower modulus of elasticity compared to those of resin-infiltrated fibrils or the demineralized dentin.

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