Mechanism of insulin-stimulated electrogenic sodium transport

Jose Rodriguez-Commes, Carlos Isales, Leena Kalghati, Jose Gasalla-Herraiz, John P. Hayslett

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Studies were performed to determine the signal transduction mechanism involved in the onset of insulin stimulated electrogenic sodium transport (Ieq) in cultured A6 cells. Insulin stimulated Ieq at a threshold concentration of one nM and a half-maximum concentration of approximately 3 nM. The onset of action occurred within 10 seconds and the increase in Ieq was augmented by pretreatment with aldosterone, similar to the action of vasopressin. Insulin stimulated an increase in Ca(i)2+ in a dose-dependent manner that involved release from intracellular stores. Hormone stimulated Ieq was dependent on increases in Ca(i)2+ because pretreatment with 5, 5' dimethyl BAPTA/AM blocked the increase in sodium transport. Further studies with dihydroxyclorpromazine, trifluoperazine and genistein, inhibitors of PKC, Ca(i)2+ dependent, calmodulin dependent kinases and tyrosine kinase, respectively, suggested that the action of insulin was dependent on activation of these kinases. In contrast, insulin stimulated Ieq was independent of changes in cAMP, because insulin did not increase the accumulation of cAMP, and inhibition of adenylate cyclase with 2', 5' dideoxyadenosine did not affect transport. These results suggest that insulin, as previously shown for aldosterone, activates apical membrane amiloride sensitive sodium channels by a calcium-dependent second messenger system.

Original languageEnglish (US)
Pages (from-to)666-674
Number of pages9
JournalKidney International
Volume46
Issue number3
DOIs
StatePublished - Sep 1994

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Sodium
Insulin
Aldosterone
Trifluoperazine
Calcium-Calmodulin-Dependent Protein Kinases
Sodium Channels
Genistein
Amiloride
Second Messenger Systems
Vasopressins
Adenylyl Cyclases
Protein-Tyrosine Kinases
Cultured Cells
Signal Transduction
Phosphotransferases
Hormones
Calcium
Membranes

ASJC Scopus subject areas

  • Nephrology

Cite this

Rodriguez-Commes, J., Isales, C., Kalghati, L., Gasalla-Herraiz, J., & Hayslett, J. P. (1994). Mechanism of insulin-stimulated electrogenic sodium transport. Kidney International, 46(3), 666-674. https://doi.org/10.1038/ki.1994.319

Mechanism of insulin-stimulated electrogenic sodium transport. / Rodriguez-Commes, Jose; Isales, Carlos; Kalghati, Leena; Gasalla-Herraiz, Jose; Hayslett, John P.

In: Kidney International, Vol. 46, No. 3, 09.1994, p. 666-674.

Research output: Contribution to journalArticle

Rodriguez-Commes, J, Isales, C, Kalghati, L, Gasalla-Herraiz, J & Hayslett, JP 1994, 'Mechanism of insulin-stimulated electrogenic sodium transport', Kidney International, vol. 46, no. 3, pp. 666-674. https://doi.org/10.1038/ki.1994.319
Rodriguez-Commes J, Isales C, Kalghati L, Gasalla-Herraiz J, Hayslett JP. Mechanism of insulin-stimulated electrogenic sodium transport. Kidney International. 1994 Sep;46(3):666-674. https://doi.org/10.1038/ki.1994.319
Rodriguez-Commes, Jose ; Isales, Carlos ; Kalghati, Leena ; Gasalla-Herraiz, Jose ; Hayslett, John P. / Mechanism of insulin-stimulated electrogenic sodium transport. In: Kidney International. 1994 ; Vol. 46, No. 3. pp. 666-674.
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