Mechanism of recovery from acute virus infection. IV. Questionable role of mononuclear phagocytes in the clearance of lymphocytic choriomeningitis virus from spleens of mice

F. Lehmann-Grube, I. Krenz, T. Krahnert, R. Schwachenwald, Dimitrios Moskofidis, J. Löhler, C. J. Villeda Posada

Research output: Contribution to journalArticle

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Abstract

After intravenous infection of mice with 103 infectious units (IU) the WE strain lymphocytic choriomeningitis (LCM) virus multiplied in the spleen (as in all other major organs), reaching more than 108 IU/g of tissue on days 4 to 5. Subsequently, the virus was quickly eliminated, being below detectability usually by day 10. During the time of virus clearance, the mononuclear phagocytes (MNP) of the spleen were activated as revealed by suppression of growth of Listeria monocytogenes and increase of cell-associated hydrolytic enzymes. In athymic nude mice, in whom the MNP system is assumed to be permanently activated, the virus replicated slightly but reproducibly less than in their euthymic counterparts. However, when the MNP were activated by Corynebacterium parvum, virus in spleens attained higher concentrations than in mice not so treated, and the rate of elimination was not altered. In mice whose MNP had been damaged by injection of dextran sulfate 500, the spleen virus titers were also increased, but the subsequent immune elimination was slightly delayed. Activation of spleen MNP was not evident at the time virus was rapidly cleared as a result of transfusion of LCM0-immune T lymphocytes. Adoptive immunization was as successful in mice that had been pretreated with γ-rays or cyclophosphamide, suggesting that replicating cells of their descendants, in particular monocytes, did not participate measurably in the process of elimination. Pretreatments of recipients with dextran sulfate 500 reduced the efficacy of transfused LCM-immune T lymphocytes, but this compound probably directly affected the cells. We interpret these findings to mean that the LCM virus in the mouse's spleen is controlled by a mechanism in which MNP do not play an essential role.

Original languageEnglish (US)
Pages (from-to)2282-2289
Number of pages8
JournalJournal of Immunology
Volume138
Issue number7
StatePublished - May 27 1987

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Lymphocytic choriomeningitis virus
Virus Diseases
Phagocytes
Spleen
Viruses
Dextran Sulfate
Nude Mice
Lymphocytic Choriomeningitis
T-Lymphocytes
Propionibacterium acnes
Mononuclear Phagocyte System
Listeria monocytogenes
Viral Load
Cyclophosphamide
Monocytes
Immunization
Injections
Enzymes
Growth
Infection

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Mechanism of recovery from acute virus infection. IV. Questionable role of mononuclear phagocytes in the clearance of lymphocytic choriomeningitis virus from spleens of mice. / Lehmann-Grube, F.; Krenz, I.; Krahnert, T.; Schwachenwald, R.; Moskofidis, Dimitrios; Löhler, J.; Villeda Posada, C. J.

In: Journal of Immunology, Vol. 138, No. 7, 27.05.1987, p. 2282-2289.

Research output: Contribution to journalArticle

Lehmann-Grube, F. ; Krenz, I. ; Krahnert, T. ; Schwachenwald, R. ; Moskofidis, Dimitrios ; Löhler, J. ; Villeda Posada, C. J. / Mechanism of recovery from acute virus infection. IV. Questionable role of mononuclear phagocytes in the clearance of lymphocytic choriomeningitis virus from spleens of mice. In: Journal of Immunology. 1987 ; Vol. 138, No. 7. pp. 2282-2289.
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