Mechanisms of Inhibition of Pyrimidine Dimer Formation in Deoxyribonucleic Acid by Acridine Dyes

B. M. Sutherland, John C. Sutherland

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Abstract

The ultraviolet (UV)-induced formation of cyclobutyl pyrimidine dimers in Escherichia coli deoxyribonucleic acid (DNA) in vitro has been investigated in terms of the mechanism of inhibition by acridine dyes, the effect on dimer yield of specific singlet and triplet quenchers, and the mechanism of dimer formation. Our results indicate that (a) energy transfer is important in dimer reduction by acridines, (b) this transfer occurs from the singlet (S1) of DNA, and (c) at room temperature triplet quenchers do not reduce dimer yield in DNA.

Original languageEnglish (US)
Pages (from-to)292-302
Number of pages11
JournalBiophysical Journal
Volume9
Issue number3
DOIs
StatePublished - Jan 1 1969

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Acridines
Pyrimidine Dimers
Coloring Agents
DNA
Energy Transfer
Escherichia coli
Temperature

ASJC Scopus subject areas

  • Biophysics

Cite this

Mechanisms of Inhibition of Pyrimidine Dimer Formation in Deoxyribonucleic Acid by Acridine Dyes. / Sutherland, B. M.; Sutherland, John C.

In: Biophysical Journal, Vol. 9, No. 3, 01.01.1969, p. 292-302.

Research output: Contribution to journalArticle

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