Microtubule disassembly enhances endothelium independent relaxation

J. L. Park, D. G. Johns, A. Melis, R. C. Webb

Research output: Contribution to journalArticle

Abstract

Cytoplasmic microtubules serve as a network by which vesicles and membrane-bounded organelles can travel. In addition, microtubules regulate cell shape and movement. In vascular smooth muscle (VSM), microtubules may play a role in regulating reactivity, as has been demonstrated by the potentiation of agonist-induced contraction in isolated rat pulmonary arteries. In this study, we tested the hypothesis that microtubule disruption enhance endothelium-independent relaxation in VSM. Tail artery rings from normotensive Sprague-Dawley rats (n=3) were placed in isolated organ baths for the measurement of isometric contractions using standard muscle bath techniques. The tail artery rings were contracted with serotonin (0.1 μM 5-HT) in physiological buffer. After maximal contractions were induced with 5-HT, VSM relaxation was mediated with sodium nitroprusside (0.1 μM). Microtubular disassembly with 10 μM nocodazole potentiated nitroprusside-mediated relaxation in isolated rat tail arteries. Before nocodazole treatment, tail arteries relaxed 72±3%. Relaxation to nitroprusside after nocodazole treatment was significantly enhanced to 89±2% (p<0.05). This study demonstrates that disassembly of microtubules enhances endothelium-independent VSM relaxation, implying that microtubules play a role in antagonizing NO-mediated relaxation in the VSM.

Original languageEnglish (US)
Pages (from-to)A994
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998

Fingerprint

Microtubules
Endothelium
Muscle
Vascular Smooth Muscle
Nocodazole
Tail
Nitroprusside
Arteries
Rats
Serotonin
Muscle Relaxation
Baths
Isometric Contraction
Cell Shape
Organelles
Pulmonary Artery
Cell Movement
Sprague Dawley Rats
Buffers
Membranes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Microtubule disassembly enhances endothelium independent relaxation. / Park, J. L.; Johns, D. G.; Melis, A.; Webb, R. C.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998, p. A994.

Research output: Contribution to journalArticle

Park, JL, Johns, DG, Melis, A & Webb, RC 1998, 'Microtubule disassembly enhances endothelium independent relaxation', FASEB Journal, vol. 12, no. 5, pp. A994.
Park, J. L. ; Johns, D. G. ; Melis, A. ; Webb, R. C. / Microtubule disassembly enhances endothelium independent relaxation. In: FASEB Journal. 1998 ; Vol. 12, No. 5. pp. A994.
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