Mineralocorticoids upregulate arterial contraction to epidermal growth factor

Jennifer A. Florian, Anne Dorrance, R. Clinton Webb, Stephanie W. Watts

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The present studies test the hypothesis that contraction to EGF is dependent on mineralocorticoids and/or an elevation in systolic blood pressure (SBP). Endothelium-denuded thoracic aortas from sham normotensive, Nω-nitro-L-arginine (L-NNA) hypertensive, Wistar-Kyoto (WKY), and spontaneously hypertensive rats (SHR) were used in isolated tissue-bath experiments. Maximal contraction to epidermal growth factor [EGF; percentage of phenylephrine (PE; 10 umol/l)-induced contraction] was greater in strips from L-NNA (32 ± 5%) and SHR (53 ± 8%) rats compared with sham and WKY rats (17 ± 1 and 12 ± 4%, respectively). Wistar-Furth rats became only mildly hypertensive when given DOCA salt (134 ± 6 mmHg) compared with Wistar rats (176 ± 9 mmHg), but aortas from both strains had a similarly enhanced contraction to EGF (∼9 times the maximal contraction of sham aorta). Furthermore, in vitro incubation of aortas from Wistar and Wistar-Furth rats with aldosterone (10 nmol/l) increased EGF-receptor mRNA expression by >50%. These data indicate that arterial contraction to EGF may occur independent of hypertension and be stimulated by mineralocorticoids.

Original languageEnglish (US)
Pages (from-to)R878-R886
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume281
Issue number3 50-3
StatePublished - Oct 2 2001

Fingerprint

Mineralocorticoids
Epidermal Growth Factor
Up-Regulation
Inbred WF Rats
Aorta
Inbred SHR Rats
Desoxycorticosterone Acetate
Blood Pressure
Inbred WKY Rats
Phenylephrine
Aldosterone
Thoracic Aorta
Baths
Epidermal Growth Factor Receptor
Endothelium
Arginine
Wistar Rats
Salts
Hypertension
Messenger RNA

Keywords

  • Hypertension
  • Vascular smooth muscle contraction
  • Wistar/Wistar-Furth rats

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Mineralocorticoids upregulate arterial contraction to epidermal growth factor. / Florian, Jennifer A.; Dorrance, Anne; Webb, R. Clinton; Watts, Stephanie W.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 281, No. 3 50-3, 02.10.2001, p. R878-R886.

Research output: Contribution to journalArticle

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