Minimal residual disease detection by flow cytometry in adult T-cell leukemia/lymphoma

Haipeng Shao, Constance M. Yuan, Liqiang Xi, Mark Raffeld, John C. Morris, John Edward Janik, Maryalice Stetler-Stevenson

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Little information exists regarding the detection of minimal residual disease (MRD) in adult T-cell leukemia/lymphoma (ATLL). We evaluated 75 peripheral blood samples from 17 ATLL cases using flow cytometry (FC); 50 of the samples were concurrently evaluated by polymerase chain reaction (PCR) for clonal T-cell receptor γ chain (TRG) gene rearrangement and the presence of human T-cell lymphotropic virus-1 proviral sequences. Residual ATLL cells were identified using a multiparametric approach to identify aberrant T-cell immunophenotypes. Malignant T cells were CD4+, CD3 dim+, CD26-, CD25 bright, CD7+, and CD27+, with occasional dim expression of CD2 or CD5. FC exhibited a high sensitivity, detecting as few as 0.29% ATLL cells/WBC (4.9 cells/μL) in the peripheral blood. PCR for TRG gene rearrangement was slightly more sensitive, and FC and PCR complemented each other in detecting MRD. In 2 patients, there was complete remission; 4 patients had disease refractory to therapy, and 3 died; 11 others had persistent disease with variable numbers of ATLL cells in the peripheral blood. Higher levels of ATLL cells appeared to correlate with disease severity. FC detection of aberrant T cells permits sensitive and quantitative monitoring of MRD in ATLL.

Original languageEnglish (US)
Pages (from-to)592-601
Number of pages10
JournalAmerican Journal of Clinical Pathology
Volume133
Issue number4
DOIs
Publication statusPublished - Apr 1 2010

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Keywords

  • Adult T-cell leukemia/lymphoma
  • CD26
  • Flow cytometry
  • HTLV-1
  • Human T-cell lymphotropic virus-1
  • Immunophenotype
  • Minimal residual disease
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Shao, H., Yuan, C. M., Xi, L., Raffeld, M., Morris, J. C., Janik, J. E., & Stetler-Stevenson, M. (2010). Minimal residual disease detection by flow cytometry in adult T-cell leukemia/lymphoma. American Journal of Clinical Pathology, 133(4), 592-601. https://doi.org/10.1309/AJCPS1K0OHLJYWWV