Minimal residual disease detection by flow cytometry in adult T-cell leukemia/lymphoma

Haipeng Shao, Constance M. Yuan, Liqiang Xi, Mark Raffeld, John C. Morris, John Edward Janik, Maryalice Stetler-Stevenson

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Little information exists regarding the detection of minimal residual disease (MRD) in adult T-cell leukemia/lymphoma (ATLL). We evaluated 75 peripheral blood samples from 17 ATLL cases using flow cytometry (FC); 50 of the samples were concurrently evaluated by polymerase chain reaction (PCR) for clonal T-cell receptor γ chain (TRG) gene rearrangement and the presence of human T-cell lymphotropic virus-1 proviral sequences. Residual ATLL cells were identified using a multiparametric approach to identify aberrant T-cell immunophenotypes. Malignant T cells were CD4+, CD3 dim+, CD26-, CD25 bright, CD7+, and CD27+, with occasional dim expression of CD2 or CD5. FC exhibited a high sensitivity, detecting as few as 0.29% ATLL cells/WBC (4.9 cells/μL) in the peripheral blood. PCR for TRG gene rearrangement was slightly more sensitive, and FC and PCR complemented each other in detecting MRD. In 2 patients, there was complete remission; 4 patients had disease refractory to therapy, and 3 died; 11 others had persistent disease with variable numbers of ATLL cells in the peripheral blood. Higher levels of ATLL cells appeared to correlate with disease severity. FC detection of aberrant T cells permits sensitive and quantitative monitoring of MRD in ATLL.

Original languageEnglish (US)
Pages (from-to)592-601
Number of pages10
JournalAmerican Journal of Clinical Pathology
Volume133
Issue number4
DOIs
StatePublished - Apr 1 2010

Fingerprint

Adult T Cell Leukemia Lymphoma
Residual Neoplasm
Flow Cytometry
T-Lymphocytes
Gene Rearrangement
Polymerase Chain Reaction
Human T-lymphotropic virus 1
T-Cell Antigen Receptor

Keywords

  • Adult T-cell leukemia/lymphoma
  • CD26
  • Flow cytometry
  • HTLV-1
  • Human T-cell lymphotropic virus-1
  • Immunophenotype
  • Minimal residual disease
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Shao, H., Yuan, C. M., Xi, L., Raffeld, M., Morris, J. C., Janik, J. E., & Stetler-Stevenson, M. (2010). Minimal residual disease detection by flow cytometry in adult T-cell leukemia/lymphoma. American Journal of Clinical Pathology, 133(4), 592-601. https://doi.org/10.1309/AJCPS1K0OHLJYWWV

Minimal residual disease detection by flow cytometry in adult T-cell leukemia/lymphoma. / Shao, Haipeng; Yuan, Constance M.; Xi, Liqiang; Raffeld, Mark; Morris, John C.; Janik, John Edward; Stetler-Stevenson, Maryalice.

In: American Journal of Clinical Pathology, Vol. 133, No. 4, 01.04.2010, p. 592-601.

Research output: Contribution to journalArticle

Shao, H, Yuan, CM, Xi, L, Raffeld, M, Morris, JC, Janik, JE & Stetler-Stevenson, M 2010, 'Minimal residual disease detection by flow cytometry in adult T-cell leukemia/lymphoma', American Journal of Clinical Pathology, vol. 133, no. 4, pp. 592-601. https://doi.org/10.1309/AJCPS1K0OHLJYWWV
Shao, Haipeng ; Yuan, Constance M. ; Xi, Liqiang ; Raffeld, Mark ; Morris, John C. ; Janik, John Edward ; Stetler-Stevenson, Maryalice. / Minimal residual disease detection by flow cytometry in adult T-cell leukemia/lymphoma. In: American Journal of Clinical Pathology. 2010 ; Vol. 133, No. 4. pp. 592-601.
@article{b1016ddd9fd04cfabe4fa5df38bb8b82,
title = "Minimal residual disease detection by flow cytometry in adult T-cell leukemia/lymphoma",
abstract = "Little information exists regarding the detection of minimal residual disease (MRD) in adult T-cell leukemia/lymphoma (ATLL). We evaluated 75 peripheral blood samples from 17 ATLL cases using flow cytometry (FC); 50 of the samples were concurrently evaluated by polymerase chain reaction (PCR) for clonal T-cell receptor γ chain (TRG) gene rearrangement and the presence of human T-cell lymphotropic virus-1 proviral sequences. Residual ATLL cells were identified using a multiparametric approach to identify aberrant T-cell immunophenotypes. Malignant T cells were CD4+, CD3 dim+, CD26-, CD25 bright, CD7+, and CD27+, with occasional dim expression of CD2 or CD5. FC exhibited a high sensitivity, detecting as few as 0.29{\%} ATLL cells/WBC (4.9 cells/μL) in the peripheral blood. PCR for TRG gene rearrangement was slightly more sensitive, and FC and PCR complemented each other in detecting MRD. In 2 patients, there was complete remission; 4 patients had disease refractory to therapy, and 3 died; 11 others had persistent disease with variable numbers of ATLL cells in the peripheral blood. Higher levels of ATLL cells appeared to correlate with disease severity. FC detection of aberrant T cells permits sensitive and quantitative monitoring of MRD in ATLL.",
keywords = "Adult T-cell leukemia/lymphoma, CD26, Flow cytometry, HTLV-1, Human T-cell lymphotropic virus-1, Immunophenotype, Minimal residual disease, Polymerase chain reaction",
author = "Haipeng Shao and Yuan, {Constance M.} and Liqiang Xi and Mark Raffeld and Morris, {John C.} and Janik, {John Edward} and Maryalice Stetler-Stevenson",
year = "2010",
month = "4",
day = "1",
doi = "10.1309/AJCPS1K0OHLJYWWV",
language = "English (US)",
volume = "133",
pages = "592--601",
journal = "American Journal of Clinical Pathology",
issn = "0002-9173",
publisher = "American Society of Clinical Pathologists",
number = "4",

}

TY - JOUR

T1 - Minimal residual disease detection by flow cytometry in adult T-cell leukemia/lymphoma

AU - Shao, Haipeng

AU - Yuan, Constance M.

AU - Xi, Liqiang

AU - Raffeld, Mark

AU - Morris, John C.

AU - Janik, John Edward

AU - Stetler-Stevenson, Maryalice

PY - 2010/4/1

Y1 - 2010/4/1

N2 - Little information exists regarding the detection of minimal residual disease (MRD) in adult T-cell leukemia/lymphoma (ATLL). We evaluated 75 peripheral blood samples from 17 ATLL cases using flow cytometry (FC); 50 of the samples were concurrently evaluated by polymerase chain reaction (PCR) for clonal T-cell receptor γ chain (TRG) gene rearrangement and the presence of human T-cell lymphotropic virus-1 proviral sequences. Residual ATLL cells were identified using a multiparametric approach to identify aberrant T-cell immunophenotypes. Malignant T cells were CD4+, CD3 dim+, CD26-, CD25 bright, CD7+, and CD27+, with occasional dim expression of CD2 or CD5. FC exhibited a high sensitivity, detecting as few as 0.29% ATLL cells/WBC (4.9 cells/μL) in the peripheral blood. PCR for TRG gene rearrangement was slightly more sensitive, and FC and PCR complemented each other in detecting MRD. In 2 patients, there was complete remission; 4 patients had disease refractory to therapy, and 3 died; 11 others had persistent disease with variable numbers of ATLL cells in the peripheral blood. Higher levels of ATLL cells appeared to correlate with disease severity. FC detection of aberrant T cells permits sensitive and quantitative monitoring of MRD in ATLL.

AB - Little information exists regarding the detection of minimal residual disease (MRD) in adult T-cell leukemia/lymphoma (ATLL). We evaluated 75 peripheral blood samples from 17 ATLL cases using flow cytometry (FC); 50 of the samples were concurrently evaluated by polymerase chain reaction (PCR) for clonal T-cell receptor γ chain (TRG) gene rearrangement and the presence of human T-cell lymphotropic virus-1 proviral sequences. Residual ATLL cells were identified using a multiparametric approach to identify aberrant T-cell immunophenotypes. Malignant T cells were CD4+, CD3 dim+, CD26-, CD25 bright, CD7+, and CD27+, with occasional dim expression of CD2 or CD5. FC exhibited a high sensitivity, detecting as few as 0.29% ATLL cells/WBC (4.9 cells/μL) in the peripheral blood. PCR for TRG gene rearrangement was slightly more sensitive, and FC and PCR complemented each other in detecting MRD. In 2 patients, there was complete remission; 4 patients had disease refractory to therapy, and 3 died; 11 others had persistent disease with variable numbers of ATLL cells in the peripheral blood. Higher levels of ATLL cells appeared to correlate with disease severity. FC detection of aberrant T cells permits sensitive and quantitative monitoring of MRD in ATLL.

KW - Adult T-cell leukemia/lymphoma

KW - CD26

KW - Flow cytometry

KW - HTLV-1

KW - Human T-cell lymphotropic virus-1

KW - Immunophenotype

KW - Minimal residual disease

KW - Polymerase chain reaction

UR - http://www.scopus.com/inward/record.url?scp=77950488307&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77950488307&partnerID=8YFLogxK

U2 - 10.1309/AJCPS1K0OHLJYWWV

DO - 10.1309/AJCPS1K0OHLJYWWV

M3 - Article

VL - 133

SP - 592

EP - 601

JO - American Journal of Clinical Pathology

JF - American Journal of Clinical Pathology

SN - 0002-9173

IS - 4

ER -