TY - JOUR
T1 - Minimal residual disease detection by flow cytometry in adult T-cell leukemia/lymphoma
AU - Shao, Haipeng
AU - Yuan, Constance M.
AU - Xi, Liqiang
AU - Raffeld, Mark
AU - Morris, John C.
AU - Janik, John Edward
AU - Stetler-Stevenson, Maryalice
PY - 2010/4
Y1 - 2010/4
N2 - Little information exists regarding the detection of minimal residual disease (MRD) in adult T-cell leukemia/lymphoma (ATLL). We evaluated 75 peripheral blood samples from 17 ATLL cases using flow cytometry (FC); 50 of the samples were concurrently evaluated by polymerase chain reaction (PCR) for clonal T-cell receptor γ chain (TRG) gene rearrangement and the presence of human T-cell lymphotropic virus-1 proviral sequences. Residual ATLL cells were identified using a multiparametric approach to identify aberrant T-cell immunophenotypes. Malignant T cells were CD4+, CD3 dim+, CD26-, CD25 bright, CD7+, and CD27+, with occasional dim expression of CD2 or CD5. FC exhibited a high sensitivity, detecting as few as 0.29% ATLL cells/WBC (4.9 cells/μL) in the peripheral blood. PCR for TRG gene rearrangement was slightly more sensitive, and FC and PCR complemented each other in detecting MRD. In 2 patients, there was complete remission; 4 patients had disease refractory to therapy, and 3 died; 11 others had persistent disease with variable numbers of ATLL cells in the peripheral blood. Higher levels of ATLL cells appeared to correlate with disease severity. FC detection of aberrant T cells permits sensitive and quantitative monitoring of MRD in ATLL.
AB - Little information exists regarding the detection of minimal residual disease (MRD) in adult T-cell leukemia/lymphoma (ATLL). We evaluated 75 peripheral blood samples from 17 ATLL cases using flow cytometry (FC); 50 of the samples were concurrently evaluated by polymerase chain reaction (PCR) for clonal T-cell receptor γ chain (TRG) gene rearrangement and the presence of human T-cell lymphotropic virus-1 proviral sequences. Residual ATLL cells were identified using a multiparametric approach to identify aberrant T-cell immunophenotypes. Malignant T cells were CD4+, CD3 dim+, CD26-, CD25 bright, CD7+, and CD27+, with occasional dim expression of CD2 or CD5. FC exhibited a high sensitivity, detecting as few as 0.29% ATLL cells/WBC (4.9 cells/μL) in the peripheral blood. PCR for TRG gene rearrangement was slightly more sensitive, and FC and PCR complemented each other in detecting MRD. In 2 patients, there was complete remission; 4 patients had disease refractory to therapy, and 3 died; 11 others had persistent disease with variable numbers of ATLL cells in the peripheral blood. Higher levels of ATLL cells appeared to correlate with disease severity. FC detection of aberrant T cells permits sensitive and quantitative monitoring of MRD in ATLL.
KW - Adult T-cell leukemia/lymphoma
KW - CD26
KW - Flow cytometry
KW - HTLV-1
KW - Human T-cell lymphotropic virus-1
KW - Immunophenotype
KW - Minimal residual disease
KW - Polymerase chain reaction
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U2 - 10.1309/AJCPS1K0OHLJYWWV
DO - 10.1309/AJCPS1K0OHLJYWWV
M3 - Article
C2 - 20231613
AN - SCOPUS:77950488307
SN - 0002-9173
VL - 133
SP - 592
EP - 601
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
IS - 4
ER -