Mitochondrial aldehyde dehydrogenase prevents ROS-induced vascular contraction in angiotensin-II hypertensive mice

Hyehun Choi, Rita C. Tostes, R Clinton Webb

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Mitochondrial aldehyde dehydrogenase (ALDH2) is an enzyme that detoxifies aldehydes to carboxylic acids. ALDH2 deficiency is known to increase oxidative stress, which is the imbalance between reactive oxygen species (ROS) generation and antioxidant defense activity. Increased ROS contribute to vascular dysfunction and structural remodeling in hypertension. We hypothesized that ALDH2 plays a protective role to reduce vascular contraction in angiotensin-II (AngII) hypertensive mice. Endothelium-denuded aortic rings from C57BL6 mice, treated with AngII (3.6 μg/kg/min, 14 days), were used to measure isometric force development. Rings treated with daidzin (10 μmol/L), an ALDH2 inhibitor, potentiated contractile responses to phenylephrine (PE) in AngII mice. Tempol (1 mmol/L) and catalase (600 U/mL) attenuated the augmented contractile effect of daidzin. In normotensive mice, contraction to PE in the presence of the daidzin was not different from control, untreated values. AngII aortic rings transfected with ALDH2 recombinant protein decreased contractile responses to PE compared with control. These data suggest that ALDH2 reduces vascular contraction in AngII hypertensive mice. Because tempol and catalase blocked the contractile response of the ALDH2 inhibitor, ROS generation by AngII may be decreased by ALDH2, thereby preventing ROS-induced contraction.

Original languageEnglish (US)
Pages (from-to)154-160
Number of pages7
JournalJournal of the American Society of Hypertension
Volume5
Issue number3
DOIs
StatePublished - May 1 2011

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Angiotensin II
Blood Vessels
Reactive Oxygen Species
Phenylephrine
Catalase
Carboxylic Acids
Recombinant Proteins
Aldehydes
Endothelium
Mitochondrial Aldehyde Dehydrogenase
Oxidative Stress
Antioxidants
Hypertension
Enzymes
daidzin
tempol

Keywords

  • ALDH2
  • Hypertension
  • ROS
  • vascular contraction

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Internal Medicine

Cite this

Mitochondrial aldehyde dehydrogenase prevents ROS-induced vascular contraction in angiotensin-II hypertensive mice. / Choi, Hyehun; Tostes, Rita C.; Webb, R Clinton.

In: Journal of the American Society of Hypertension, Vol. 5, No. 3, 01.05.2011, p. 154-160.

Research output: Contribution to journalArticle

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