Modulated human globin gene expression: Role for antisense expression vectors

L. Xu, B. S. Pace

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Several approaches have been explored to prevent polymerization of sickle hemoglobin in erythrocytes. We tested the ability of a mammalian expression vector carrying a β gene antisense cDNA fragment to block β gene expression. The antisense expression vector was stably transfected into HS2γβ stable mouse erythroleukemia cell lines producing human γ and β globin chains. By day 14 there was an average 24% decrease and 12% increase in β and γ globin mRNA levels respectively. We observed a loss of β gene inhibition by day 42. This study suggests that a β globin antisense cDNA expression vector may be an alternative gene therapy strategy to decrease sickle hemoglobin levels in patient with sickle cell disease.

Original languageEnglish (US)
Pages (from-to)423-433
Number of pages11
JournalCellular and Molecular Biology Letters
Volume3
Issue number4
StatePublished - Jan 1 1998
Externally publishedYes

Keywords

  • Antisense Expression Vector
  • Oligodeoxynucleotides
  • β globin
  • γ globin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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