Modulated human globin gene expression: Role for antisense expression vectors

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Several approaches have been explored to prevent polymerization of sickle hemoglobin in erythrocytes. We tested the ability of a mammalian expression vector carrying a β gene antisense cDNA fragment to block β gene expression. The antisense expression vector was stably transfected into HS2γβ stable mouse erythroleukemia cell lines producing human γ and β globin chains. By day 14 there was an average 24% decrease and 12% increase in β and γ globin mRNA levels respectively. We observed a loss of β gene inhibition by day 42. This study suggests that a β globin antisense cDNA expression vector may be an alternative gene therapy strategy to decrease sickle hemoglobin levels in patient with sickle cell disease.

Original languageEnglish (US)
Pages (from-to)423-433
Number of pages11
JournalCellular and Molecular Biology Letters
Volume3
Issue number4
StatePublished - Jan 1 1998
Externally publishedYes

Fingerprint

Globins
Gene expression
Sickle Hemoglobin
Gene Expression
Complementary DNA
Genes
Gene therapy
Leukemia, Erythroblastic, Acute
Sickle Cell Anemia
Complementary Therapies
Polymerization
Genetic Therapy
Erythrocytes
Cells
Cell Line
Messenger RNA

Keywords

  • Antisense Expression Vector
  • Oligodeoxynucleotides
  • β globin
  • γ globin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Modulated human globin gene expression : Role for antisense expression vectors. / Xu, L.; Pace, Betty Sue.

In: Cellular and Molecular Biology Letters, Vol. 3, No. 4, 01.01.1998, p. 423-433.

Research output: Contribution to journalArticle

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