Modulation in the microRNA repertoire is responsible for the stage-specific effects of Akt suppression on murine neuroendocrine prostate cancer

Abdulrahman Alwhaibi, Fei Gao, Sandeep Artham, Bernard M. Hsia, Ashis Mondal, Ravindra Kolhe, Payaningal R. Somanath

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Recent studies indicate a stage-specific, differential role for the oncogene Akt on various cancers. In prostate cancer (PCa), suppression of Akt activity in the advanced stages promoted transforming growth factor-β (TGFβ) pathway-mediated epithelial-to-mesenchymal transition (EMT) and metastasis to the lungs. In the current study, we performed Affymetrix analysis to compare the expression profile of microRNAs in the mouse prostate tissues collected at the prostatic inter-epithelial neoplasia (PIN) stage from Transgenic adenocarcinoma of the mouse (TRAMP)/Akt1+/+ versus TRAMP/Akt1–/– mice, and at the advanced stage from TRAMP/Akt1+/+ mice treated with triciribine (Akt inhibitor) versus DMSO-treated control. Our analysis demonstrates that in the early stage, Akt1 in the TRAMP prostate tumors express a set of miRNAs responsible for regulating cancer cell survival, proliferation, and tumor growth, whereas, in the advanced stages, a different set of miRNAs that promote EMT and cancer metastasis is expressed. Our study has identified novel Akt-regulated signature microRNAs in the early and advanced PCa and demonstrates their differential effects on PCa growth and metastasis.

Original languageEnglish (US)
Article numbere00796
JournalHeliyon
Volume4
Issue number9
DOIs
StatePublished - Sep 2018

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MicroRNAs
Prostatic Neoplasms
Neoplasms
Epithelial-Mesenchymal Transition
triciribine
Neoplasm Metastasis
Prostate
Transforming Growth Factors
Growth
Dimethyl Sulfoxide
Oncogenes
Transgenic Mice
Cell Survival
Adenocarcinoma
Cell Proliferation
Lung

Keywords

  • Biochemistry
  • Bioinformatics
  • Cancer research

ASJC Scopus subject areas

  • General

Cite this

Modulation in the microRNA repertoire is responsible for the stage-specific effects of Akt suppression on murine neuroendocrine prostate cancer. / Alwhaibi, Abdulrahman; Gao, Fei; Artham, Sandeep; Hsia, Bernard M.; Mondal, Ashis; Kolhe, Ravindra; Somanath, Payaningal R.

In: Heliyon, Vol. 4, No. 9, e00796, 09.2018.

Research output: Contribution to journalArticle

Alwhaibi, Abdulrahman ; Gao, Fei ; Artham, Sandeep ; Hsia, Bernard M. ; Mondal, Ashis ; Kolhe, Ravindra ; Somanath, Payaningal R. / Modulation in the microRNA repertoire is responsible for the stage-specific effects of Akt suppression on murine neuroendocrine prostate cancer. In: Heliyon. 2018 ; Vol. 4, No. 9.
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