Modulation of endothelial cell nitric oxide synthase expression

David G. Harrison, Nobutaka Inoue, Yuichi Ohara, Tohru Fukai

Research output: Contribution to journalReview article

14 Scopus citations

Abstract

The 5' promoter region of endothelial cell nitric oxide synthase (ecNOS) gene has several features which are compatible with a constitutively expressed, so called 'housekeeping' gone. These include absence of a TATA box and the presence of Sp1 binding sties situated near the transcription start site. The promoter also contains sequences which suggest that it may be regulated by a variety of transcription factor-mediated signals. Studies of cultured endothelial cells show that ecNOS expression is modulated by shear stress, transforming growth factor beta, inhibition of protein kinase C, and the state of proliferation. These experiments indicate that although the ecNOS is a 'constitutively expressed' gene, its content in the endothelium is subject to modest degrees of regulation which may have important physiological and pathophysiological implications.

Original languageEnglish (US)
Pages (from-to)815-821
Number of pages7
JournalJAPANESE CIRCULATION JOURNAL
Volume60
Issue number11
DOIs
StatePublished - Nov 1996
Externally publishedYes

Keywords

  • Gene expression
  • Nitric oxide synthase
  • Protein kinase C
  • Shear stress
  • Transforming growth factor β

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

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