Molecular cloning and primary structure of Man9‐mannosidase from human kidney

Ernst BAUSE, Erhard Bieberich, Andreas ROLFS, Christof VÖLKER, Bernhard SCHMIDT

Research output: Contribution to journalArticle

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Abstract

Man9‐mannosidase, a processing enzyme found in the endoplasmic reticulum (ER), catalyses the removal of three distinct mannose residues from peptide‐bound Man9‐GlcNAc2 oligosaccharides producing a single Man6 isomer [Bause, E., Breuer, W., Schweden, J., Roesser, R. & Geyer, R. (1992) Eur. J. Biochem. 208, 451–457]. We have isolated four Man9‐mannosidase‐specific clones from a human kidney cDNA library and used these to construct a full‐length cDNA of 3250 base pairs. A single open reading frame of 1875 nucleotides encodes a protein of approximately 71 kDa, consistent with data from immunological studies. Analysis of the coding sequence predicts that Man9‐mannosidase is a type II transmembrane protein consisting of a short cytoplasmic polypeptide tail, a single transmembrane domain acting as a non‐cleavable signal sequence and a large luminal catalytic domain. This domain architecture closely resembles that of other ER and Golgi‐located processing enzymes, pointing to common structural motifs involved in membrane insertion and topology. The protein sequence of the Man9‐mannosidase contains three potential N‐glycosylation sites of which only one site is used. The amino acid sequence of several peptide regions, including a calcium‐binding consensus sequence, bears striking similarities to an ER α‐1,2‐mannosidase from yeast, whereas, by contrast, no sequence similarity was detectable with rat liver ER α‐mannosidase and Golgi α‐mannosidase II. This finding may indicate that the mammalian α‐mannosidases, which differ significantly in their substrate specificity, are coded for by evolutionarily unrelated genes, providing an attractive means of regulation and fine‐tuning oligosaccharide processing, not only at the enzymic but also at the transcriptional level.

Original languageEnglish (US)
Pages (from-to)535-540
Number of pages6
JournalEuropean Journal of Biochemistry
Volume217
Issue number2
DOIs
StatePublished - Jan 1 1993

Fingerprint

Cloning
Molecular Cloning
Mannosidases
Endoplasmic Reticulum
Oligosaccharides
Kidney
Processing
Peptides
Proteins
Enzymes
Mannose
Protein Sorting Signals
Gene Library
Isomers
Liver
Yeast
Rats
Consensus Sequence
Nucleotides
Complementary DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Molecular cloning and primary structure of Man9‐mannosidase from human kidney. / BAUSE, Ernst; Bieberich, Erhard; ROLFS, Andreas; VÖLKER, Christof; SCHMIDT, Bernhard.

In: European Journal of Biochemistry, Vol. 217, No. 2, 01.01.1993, p. 535-540.

Research output: Contribution to journalArticle

BAUSE, Ernst ; Bieberich, Erhard ; ROLFS, Andreas ; VÖLKER, Christof ; SCHMIDT, Bernhard. / Molecular cloning and primary structure of Man9‐mannosidase from human kidney. In: European Journal of Biochemistry. 1993 ; Vol. 217, No. 2. pp. 535-540.
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