Multiple dendritic cell (DC) subpopulations in human gingiva and association of mature DCs with CD4+ T-cells in situ

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Gingival epithelium is a site of active trafficking of Langerhans cells (LCs), while the lamina propria in chronic periodontitis (CP) contains CD83+ mature dendritic cells (mDCs) and CD4+ T-cells. The immune cells that contribute to the mDCs, and whether mDCs engage with T-cells in situ, are unclear. Using several immunohistochemical approaches, combined with fluorescence-, light-, and scanning laser confocal-microscopy, we show that, in addition to LCs, the gingiva contains dermal DCs (DDCs) in the lamina propria; moreover, DDCs increase in number during CP. Furthermore, DDCs, LCs, and B-cells co-express CD83 in CP and contribute to the mDC pool. Double-staining for CD83 and CD4 revealed that mDCs associate with clusters of CD4+ T-cells in the lamina propria. Analysis of these data suggests that multiple DC subsets mature in the gingiva and that mature DCs engage in antigen presentation with T-cells in chronic periodontitis.

Original languageEnglish (US)
Pages (from-to)736-741
Number of pages6
JournalJournal of Dental Research
Volume82
Issue number9
DOIs
StatePublished - Jan 1 2003
Externally publishedYes

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Gingiva
Chronic Periodontitis
Dendritic Cells
Langerhans Cells
T-Lymphocytes
Skin
Mucous Membrane
Antigen Presentation
Confocal Microscopy
Catalytic Domain
B-Lymphocytes
Epithelium
Fluorescence
Staining and Labeling
Light

Keywords

  • Dendritic cells
  • Dermal DCs
  • Immunohistochemistry
  • Langerhans cells
  • Periodontitis
  • T-cells

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Multiple dendritic cell (DC) subpopulations in human gingiva and association of mature DCs with CD4+ T-cells in situ. / Jotwani, R.; Cutler, Christopher W.

In: Journal of Dental Research, Vol. 82, No. 9, 01.01.2003, p. 736-741.

Research output: Contribution to journalArticle

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