Murine monoclonal anti-DNA antibodies bind directly to glomerular antigens and form immune deposits

M. P. Madaio, J. Carlson, J. Cataldo, A. Ucci, P. Migliorini, O. Pankewycz

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201 Scopus citations

Abstract

The capacity of monoclonal anti-DNA antibodies, derived spontaneously from MRL-lpr/lpr mice, to bind directly to intrinsic glomerular antigens and form immune deposits was evaluated. Two antibodies, H130 (IgM-κ) and H241 (IgG2a-κ), bound to normal glomeruli in vitro. This binding was not inhibited by DNAase, but it was, in the case of H130, inhibited by the anti-idiotype anti-H130. Both antibodies also bound to glomerular digests on nitrocellulose. After i.v. injection, however, H241 bound to glomeruli and formed glomerular immune deposits, whereas H130 did not. Similarly, after i.p. injection of H241 hybridomas to normal mice, all mice developed glomerular immune deposits. In contrast, administration of H130 hybridomas, other anti-DNA-producing hybridomas, and other unrelated hybridomas did not lead to glomerular immune deposit formation. We conclude that certain lupus autoantibodies can form glomerular immune deposits by binding directly to non-DNA antigenic structures that are normally present in extracellular locations within normal glomeruli.

Original languageEnglish (US)
Pages (from-to)2883-2889
Number of pages7
JournalJournal of Immunology
Volume138
Issue number9
StatePublished - Jul 14 1987

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Madaio, M. P., Carlson, J., Cataldo, J., Ucci, A., Migliorini, P., & Pankewycz, O. (1987). Murine monoclonal anti-DNA antibodies bind directly to glomerular antigens and form immune deposits. Journal of Immunology, 138(9), 2883-2889.