Myocardin is sufficient for a smooth muscle-like contractile phenotype

Xiaochun Long, Robert D. Bell, William T. Gerthoffer, Berislav V. Zlokovic, Joseph M. Miano

Research output: Contribution to journalArticle

Abstract

Background - Myocardin (Myocd) is a strong coactivator that binds the serum response factor (SRF) transcription factor over CArG elements embedded within smooth muscle cell (SMC) and cardiac muscle cyto-contractile genes. Here, we sought to ascertain whether Myocd-mediated gene expression confers a structural and physiological cardiac or SMC phenotype. Methods and Results - Adenoviral-mediated expression of Myocd in the BC3H1 cell line induces cardiac and SMC genes while suppressing both skeletal muscle markers and cell growth. Immunofluorescence microscopy shows that SRF and a SMC-like cyto-contractile apparatus are elevated with Myocd overexpression. A short hairpin RNA to Srf impairs BC3H1 cyto-architecture; however, cotransduction with Myocd results in complete restoration of the cyto-architecture. Electron microscopic studies demonstrate a SMC ultrastructural phenotype with no evidence for cardiac sarcomerogenesis. Biochemical and time-lapsed videomicroscopy assays reveal clear evidence for Myocd-induced SMC-like contraction. Conclusion - Myocd is sufficient for the establishment of a SMC-like contractile phenotype.

Original languageEnglish (US)
Pages (from-to)1505-1510
Number of pages6
JournalArteriosclerosis, thrombosis, and vascular biology
Volume28
Issue number8
DOIs
StatePublished - Aug 1 2008
Externally publishedYes

Fingerprint

Smooth Muscle Myocytes
Smooth Muscle
Phenotype
Serum Response Factor
Cardiac Myocytes
Video Microscopy
myocardin
Fluorescence Microscopy
Muscle Cells
Small Interfering RNA
Genes
Myocardium
Skeletal Muscle
Transcription Factors
Electrons
Gene Expression
Cell Line
Growth

Keywords

  • Contraction
  • Knockdown
  • Myocardin
  • Serum response factor
  • Smooth muscle

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Myocardin is sufficient for a smooth muscle-like contractile phenotype. / Long, Xiaochun; Bell, Robert D.; Gerthoffer, William T.; Zlokovic, Berislav V.; Miano, Joseph M.

In: Arteriosclerosis, thrombosis, and vascular biology, Vol. 28, No. 8, 01.08.2008, p. 1505-1510.

Research output: Contribution to journalArticle

Long, Xiaochun ; Bell, Robert D. ; Gerthoffer, William T. ; Zlokovic, Berislav V. ; Miano, Joseph M. / Myocardin is sufficient for a smooth muscle-like contractile phenotype. In: Arteriosclerosis, thrombosis, and vascular biology. 2008 ; Vol. 28, No. 8. pp. 1505-1510.
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AU - Miano, Joseph M.

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