NETROPSIN

INTERACTION WITH ULTRAVIOLET IRRADIATED DNA

John C. Sutherland, John F. Duval, William H. Farland, Kathleen Pietruszka Griffin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Abstract—Ultraviolet irradiation of double‐stranded DNA reduces the circular dichroism (i < 300 nm) induced when the basic peptide antibiotic netropsin (Nt) is added to DNA subsequent to thc irradiation compared to the CD induced by the same concentrations of Nt added to unirradiated DNA. Nt is known to bind to A T base pairs in duplex DNA but will not bind to single‐stranded DNA. The reduction in the maximum induced CD observed with saturating concentrations of Nt is a linear function of the concentration of pyrimidine dimers which. along with other dinucleotide photoproducts. form short disrupted regions in duplex DNA. The decrease in the CD of Nt bound to irradiated DNA could be due to elimination of potential Nt sites in the vicinity of a dimer. reduction in the average magnitude of the CD of Nt bound near a dimer or various combinations of these effects. In addition there is a reduction in the average binding constant for Nt bound to irradiated DNA compared to unirradiated DNA suggesting that formation of dinucleotide photoproducts either tends to preferentially eliminate the tighter binding sites or that tighter sites are converted to weaker ones. A simple model suggests that no more than one‐third to one‐half of the pyrimidine dimcrs formed in DNA completely eliminate a Nt site.

Original languageEnglish (US)
Pages (from-to)943-949
Number of pages7
JournalPhotochemistry and Photobiology
Volume29
Issue number5
DOIs
StatePublished - Jan 1 1979
Externally publishedYes

Fingerprint

Netropsin
deoxyribonucleic acid
DNA
interactions
dimers
pyrimidines
Dimers
Irradiation
Pyrimidine Dimers
Circular DNA
irradiation
antibiotics
Circular Dichroism
Base Pairing
dichroism
peptides
elimination
Binding Sites

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry
  • Physical and Theoretical Chemistry

Cite this

NETROPSIN : INTERACTION WITH ULTRAVIOLET IRRADIATED DNA. / Sutherland, John C.; Duval, John F.; Farland, William H.; Griffin, Kathleen Pietruszka.

In: Photochemistry and Photobiology, Vol. 29, No. 5, 01.01.1979, p. 943-949.

Research output: Contribution to journalArticle

Sutherland, John C. ; Duval, John F. ; Farland, William H. ; Griffin, Kathleen Pietruszka. / NETROPSIN : INTERACTION WITH ULTRAVIOLET IRRADIATED DNA. In: Photochemistry and Photobiology. 1979 ; Vol. 29, No. 5. pp. 943-949.
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