Neuritogenesis, Not Receptor Expression, of NG108-15 Cells Can be Modulated by Monosialoganglioside GM1

H. M. Hwang, S. C. Weng, S. K. Lo, Robert K Yu, W. H. Tsai

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In this study, involvement of gangliosides in neurite outgrowth and receptor expression of the neuroblastoma X glioma hybrid NG108-15 cloned cells was investigated. Monosialoganglioside GM1 (100 μM) and disialoganglioside GD1a (100 μM) were applied to the culture medium at different concentrations of fetal bovine serum, 1-10%, with or without addition of dibutyryl adenosine 3′,5′-cyclic monophosphate (500 μM). In some experiments, 5 mg/ml of cholera toxin B was added to the media to block endogenous GM1. The results indicated that GM1 had an influence on cell proliferation and neuritogenesis but did not induce muscarinic receptor expression of NG108-15 cells.

Original languageEnglish (US)
Pages (from-to)211-217
Number of pages7
JournalChinese Journal of Physiology
Volume39
Issue number4
StatePublished - Dec 1 1996
Externally publishedYes

Fingerprint

Gangliosides
Cholera Toxin
Muscarinic Receptors
Neuroblastoma
Glioma
Adenosine
Culture Media
Cell Proliferation
Serum
sialogangliosides
Neuronal Outgrowth

Keywords

  • cAMP
  • Differentiation
  • Muscarinic receptor
  • Neurotrophic factor
  • Proliferation

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Neuritogenesis, Not Receptor Expression, of NG108-15 Cells Can be Modulated by Monosialoganglioside GM1. / Hwang, H. M.; Weng, S. C.; Lo, S. K.; Yu, Robert K; Tsai, W. H.

In: Chinese Journal of Physiology, Vol. 39, No. 4, 01.12.1996, p. 211-217.

Research output: Contribution to journalArticle

Hwang, H. M. ; Weng, S. C. ; Lo, S. K. ; Yu, Robert K ; Tsai, W. H. / Neuritogenesis, Not Receptor Expression, of NG108-15 Cells Can be Modulated by Monosialoganglioside GM1. In: Chinese Journal of Physiology. 1996 ; Vol. 39, No. 4. pp. 211-217.
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