Neuroadaptations in ionotropic and metabotropic glutamate receptor mRNA produced by cocaine treatment

M. Behnam Ghasemzadeh, Linda C. Nelson, Xinyun Lu, Peter W. Kalivas

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

The expression of glutamate receptor/subunit mRNAs was examined 3 weeks after discontinuing 1 week of daily injections of saline or cocaine. The level of mRNA for GluR1-4, NMDAR1, and mGluR5 receptors was measured with in situ hybridization and RT-PCR. In nucleus accumbens, acute cocaine treatment significantly reduced the mRNA level for GluR3, GluR4, and NMDAR1 subunits, whereas repeated cocaine reduced the level for GluR3 mRNA. Acute cocaine treatment also reduced the NMDAR1 mRNA level in dorsolateral striatum and ventral tegmental area. In prefrontal cortex, repeated cocaine treatment significantly increased the level of GluR2 mRNA. The GluR2 mRNA level was not changed by acute or repeated cocaine in any other brain regions examined. Repeated cocaine treatment also significantly increased mGluR5 mRNA levels in nucleus accumbens shell and dorsolateral striatum. Functional properties of the ionotropic glutamate receptors are determined by subunit composition. In addition, metabotropic glutamate receptors can modulate synaptic transmission and the response to stimulation of ionotropic receptors. Thus, the observed changes in levels of AMPA and NMDA receptor subunits and the mGluR5 metabotropic receptor may alter excitatory neurotransmission in the mesocorticolimbic dopamine system, which could play a significant role in the enduring biochemical and behavioral effects of cocaine.

Original languageEnglish (US)
Pages (from-to)157-165
Number of pages9
JournalJournal of Neurochemistry
Volume72
Issue number1
DOIs
StatePublished - Jan 11 1999

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Ionotropic Glutamate Receptors
Metabotropic Glutamate Receptors
Cocaine
Messenger RNA
Nucleus Accumbens
Therapeutics
Synaptic Transmission
Ventral Tegmental Area
AMPA Receptors
Glutamate Receptors
Prefrontal Cortex
N-Methyl-D-Aspartate Receptors
In Situ Hybridization
Dopamine
Brain
Polymerase Chain Reaction
Injections

Keywords

  • Cocaine
  • Glutamate receptor
  • Nucleus accumbens
  • Prefrontal cortex
  • Striatum
  • Ventral tegmental area

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Neuroadaptations in ionotropic and metabotropic glutamate receptor mRNA produced by cocaine treatment. / Ghasemzadeh, M. Behnam; Nelson, Linda C.; Lu, Xinyun; Kalivas, Peter W.

In: Journal of Neurochemistry, Vol. 72, No. 1, 11.01.1999, p. 157-165.

Research output: Contribution to journalArticle

Ghasemzadeh, M. Behnam ; Nelson, Linda C. ; Lu, Xinyun ; Kalivas, Peter W. / Neuroadaptations in ionotropic and metabotropic glutamate receptor mRNA produced by cocaine treatment. In: Journal of Neurochemistry. 1999 ; Vol. 72, No. 1. pp. 157-165.
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